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PMID: 9023335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional repression at a distance through exclusion of activator binding in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 3 ·1997-02-04 ·Pages 790-5

Shimizu M, Li W, Shindo H, Mitchell AP

Abstract

The yeast repressor Rme1p acts from distant binding sites to block transcription of the chromosomal IME1 gene. Rme1p can also repress the heterologous CYC1 promoter when Rme1p binding sites are placed 250-300 bp upstream of CYC1 transcriptional activator binding sites (UAS1 and UAS2). Here, in vivo footprinting studies indicate that Rme1p acts over this distance by preventing the binding of the CYC1 transcriptional activators to UAS1 and UAS2. Inhibition of activator binding by Rme1p has the same genetic requirements as repression: both depend upon sequences flanking the Rme1p binding sites and upon Rgr1p and Sin4p, two subunits of the RNA polymerase II-associated Mediator complex that are required for normal nucleosome density. Thus Rme1p may alter chromatin to prevent binding of transcriptional activators to distant DNA sequences.

MeSH Terms
CCAAT-Binding Factor Carbon-Oxygen Lyases Cytochrome c Group/genetics Cytochromes c DNA Footprinting DNA, Fungal/genetics,metabolism DNA-(Apurinic or Apyrimidinic Site) Lyase Fungal Proteins/metabolism Gene Expression Regulation, Fungal/physiology Mutation Nuclear Proteins/metabolism Promoter Regions, Genetic/genetics Repressor Proteins/metabolism Saccharomyces cerevisiae Proteins Trans-Activators/metabolism Transcription Factors/metabolism Transcription, Genetic/physiology Yeasts/genetics
Chemicals
CCAAT-Binding Factor CYC1 protein, S cerevisiae Cytochrome c Group DNA, Fungal Fungal Proteins Nuclear Proteins RME1 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Cytochromes c Carbon-Oxygen Lyases DNA-(Apurinic or Apyrimidinic Site) Lyase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shimizu M
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan. [email protected]
Li W
Shindo H
Mitchell A P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.3.790
Email
[email protected]
Published
1997-02-04
Pages
790-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19592
Subset
IM
Grants
NIGMS NIH HHS · R01 GM039531 · United States
NIGMS NIH HHS · GM39531 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.3.790 PMC Full Text View journal details
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