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PMID: 9024689 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An alpha-helical signal in the cytosolic domain of the interleukin 2 receptor beta chain mediates sorting towards degradation after endocytosis.

The Journal of cell biology ·Vol. 136 ·No. 3 ·1997-02-10 ·Pages 583-95

Subtil A, Delepierre M, Dautry-Varsat A

Abstract

High-affinity IL2 receptors consist of three components, the alpha, beta, and gamma chains that are associated in a noncovalent manner. Both the beta and gamma chains belong to the cytokine receptor superfamily. Interleukin 2 (IL2) binds to high-affinity receptors on the cell surface and IL2-receptor complexes are internalized. After endocytosis, the components of this multimolecular receptor have different intracellular fates: one of the chains, alpha, recycles to the plasma membrane, while the others, beta and gamma, are routed towards late endocytic compartments and are degraded. We show here that the cytosolic domain of the beta chain contains a 10-amino acid sequence which codes for a sorting signal. When transferred to a normally recycling receptor, this sequence diverts it from recycling. The structure of a 17-amino acid segment of the beta chain including this sequence has been studied by nuclear magnetic resonance and circular dichroism spectroscopy, which revealed that the 10 amino acids corresponding to the sorting signal form an amphipathic alpha helix. This work thus describes a novel, highly structured signal, which is sufficient for sorting towards degradation compartments after endocytosis.

MeSH Terms
Amino Acid Sequence Amino Acids Cell Membrane/metabolism Clathrin/metabolism Cytosol Endocytosis/physiology HeLa Cells Helix-Turn-Helix Motifs Humans Molecular Sequence Data Receptors, Interleukin-2/chemistry,metabolism Signal Transduction/physiology Structure-Activity Relationship Time Factors Transferrin/metabolism Tumor Cells, Cultured
Chemicals
Amino Acids Clathrin Receptors, Interleukin-2 Transferrin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Subtil A
Unité de Biologie des Interactions Cellulaires, URA CNRS 1960, Paris, France.
Delepierre M
Dautry-Varsat A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.136.3.583
Published
1997-02-10
Pages
583-95
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2134293
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.136.3.583 PMC Full Text View journal details
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