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PMID: 903155 Published · ppublish English Journal Article

Activation of mouse ribosomal RNA genes at the 2-cell stage.

Human genetics ·Vol. 38 ·No. 1 ·1977-08-31 ·Pages 57-63

Engel W, Zenzes MT, Schmid M

Abstract

The Ag-AS method, developed by Goodpasture and Bloom (1975) strains transcriptionally active nucleolus organizer regions (NORs) on the chromosomes and in the interphase nuclei. Metaphases and interphase nuclei of early mouse embryos (unfertilized eggs, pronucleus stages, 2-, 4-, 8-cell stages, and morulae) were subjected to silver-staining. First staining of a single chromosome bearing an NOR was observed at the 2-cell stage. At the 4-cell stage 4--6 chromosomes, and at the 8-cell stage invariably all the 6 chromosomes known to bear NORs, respond positively to silver-staining. These results indicate that during mouse embryogenesis ribosomal RNA genes start to function at the 2-cell stage. The polar body does not respond to silver-staining, which supports the view that the polar body genome remains inactive.

MeSH Terms
Animals Female Genes Mice/embryology RNA, Ribosomal Time Factors Zygote/physiology
Chemicals
RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Engel W
Zenzes M T
Schmid M
References (33)
33 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00295808
Published
1977-08-31
Pages
57-63
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00295808 View journal details
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