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PMID: 9037056 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Allergic airway sensitization induces T cell activation but not airway hyperresponsiveness in B cell-deficient mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 4 ·1997-02-18 ·Pages 1350-5

Hamelmann E, Vella AT, Oshiba A, Kappler JW, Marrack P, Gelfand EW

Abstract

B cells play an important role in the allergic response by producing allergen-specific Igs as well as by serving as antigen-presenting cells. We studied the involvement of B cells in the development of responses in a murine model of allergic airway sensitization. Normal and B cell-deficient (muMt-/-) B10.BR mice were sensitized via the airways to ovalbumin; Ig production, cytokine elaboration from local lymph node cells, development of airway hyperresponsiveness, and histological changes in the airways were evaluated. Both strains of mice had increased production of T helper 2-like cytokines and developed an accumulation of eosinophils in the bronchial tissue after airway sensitization. However, only wild-type mice produced allergen-specific antibodies and exhibited altered airway function. B cell-deficient mice reconstituted with anti-ovalbumin IgE during the course of sensitization developed increases in airway responsiveness. These results indicated that neither B cells nor IgE were necessary for the induction of a T helper 2-type cytokine response or eosinophil infiltration of the airways after allergic sensitization but that IgE was required as a second signal for the development of airway hyperresponsiveness in this model of airway sensitization.

MeSH Terms
Animals Antibody Specificity B-Lymphocytes/immunology Cytokines/biosynthesis Eosinophils/cytology Female Immunization Immunoglobulin E/blood Immunoglobulin G/blood Lung/cytology Lymph Nodes/cytology,metabolism Lymphocyte Activation Lymphocytes/immunology Mice Mice, Mutant Strains Ovalbumin/immunology Respiratory Hypersensitivity/immunology T-Lymphocytes/immunology Trachea/immunology
Chemicals
Cytokines Immunoglobulin G Immunoglobulin E Ovalbumin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hamelmann E
Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA.
Vella A T
Oshiba A
Kappler J W
Marrack P
Gelfand E W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.4.1350
Published
1997-02-18
Pages
1350-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19794
Subset
IM
Grants
NHLBI NIH HHS · P01 HL036577 · United States
NIAID NIH HHS · AI-29704 · United States
NHLBI NIH HHS · HL-36577 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.4.1350 PMC Full Text View journal details
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