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PMID: 9041430 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Dot blot assay for the serotyping of pneumococci.

Journal of clinical microbiology ·Vol. 35 ·No. 3 ·1997-03-00 ·Pages 764-6

Fenoll A, Jado I, Vicioso D, Casal J

Abstract

To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumococci have to be studied.

MeSH Terms

Antibodies, Bacterial Cross Reactions Evaluation Studies as Topic Humans Immunoblotting/methods,statistics & numerical data Pneumococcal Infections/microbiology Sensitivity and Specificity Serotyping/methods,statistics & numerical data Streptococcus pneumoniae/classification,immunology,isolation & purification

Chemicals

Antibodies, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fenoll A

Laboratorio de Referencia de Neumococos, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Jado I

Vicioso D

Casal J

References (6)

6 references, click to expand

Capillary precipitin typing of Streptococcus pneumoniae.
J Clin Microbiol. 1978 Oct;8(4):355-9 PMID: 31369
Comparison of counterimmunoelectrophoresis and the capsular reaction test for typing of pneumococci.
J Clin Microbiol. 1980 Jun;11(6):589-92 PMID: 7430329
Antimicrobial resistance in Streptococcus pneumoniae: an overview.
Clin Infect Dis. 1992 Jul;15(1):77-83 PMID: 1617076
An alternative approach to typing of Streptococcus pneumoniae strains by coagglutination.
Acta Pathol Microbiol Immunol Scand B. 1986 Dec;94(6):409-13 PMID: 3565015
Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolates causing systemic infections in Spain, 1979-1989.
Rev Infect Dis. 1991 Jan-Feb;13(1):56-60 PMID: 2017632
Serotyping of pneumococci by polyvalent counterimmunoelectrophoresis.
J Hosp Infect. 1985 Mar;6(1):110-1 PMID: 2859314

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.35.3.764-766.1997

Published

1997-03-00

Pages

764-6

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC229668

Subset

External Links

PubMed source DOI: 10.1128/jcm.35.3.764-766.1997 PMC Full Text View journal details

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Dot blot assay for the serotyping of pneumococci.

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