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PMID: 9048880 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and expression of a novel Staphylococcus aureus antigen.

Biochimica et biophysica acta ·Vol. 1350 ·No. 2 ·1997-02-07 ·Pages 128-32

Rieneck K, Renneberg J, Diamant M, Gutschik E, Bendtzen K

Abstract

A novel gene encoding an antigen from Staphylococcus aureus was isolated from an expression library by screening with antisera from patients with deep Staphylococcus aureus infections. In one positive clone an open reading frame, named ORF-2, was identified. Recombinant ORF-2 protein reacted with human immune serum. ORF-2 was shown to be present in other Staphylococcus aureus strains, but not in related species.

MeSH Terms
Amino Acid Sequence Antibodies, Bacterial Antigens, Bacterial/genetics Bacterial Proteins/genetics,immunology Base Sequence Cloning, Molecular DNA Primers/genetics DNA, Bacterial/genetics Gene Expression Genes, Bacterial Humans Molecular Sequence Data Open Reading Frames Recombinant Proteins/genetics,immunology Sequence Homology, Amino Acid Staphylococcal Infections/immunology Staphylococcus aureus/genetics,immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins DNA Primers DNA, Bacterial Recombinant Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rieneck K
Institute for Inflammation Research, Laboratory of Medical Immunology, TA 7544, Rigshospitalet, Copenhagen, N. Denmark. [email protected]
Renneberg J
Diamant M
Gutschik E
Bendtzen K
Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
Published
1997-02-07
Pages
128-32
Language
English
Region
Netherlands
NLM ID
0217513
Subset
IM
Databases
GENBANK
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