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PMID: 9049254 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB), a neural member of the EGF family of differentiation factors, is implicated in neurite formation.

The Journal of cell biology ·Vol. 136 ·No. 4 ·1997-02-24 ·Pages 895-906

Schumacher S, Volkmer H, Buck F, Otto A, Tarnók A, Roth S, Rathjen FG

Abstract

Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB) was identified by combining binding assays with immunological screens in the chicken nervous system as a novel member of the EGF family of differentiation factors. cDNA cloning indicates that CALEB is a multidomain protein that consists of an NH2-terminal glycosylation region, a leucine-proline-rich segment, an acidic box, a single EGF-like domain, a transmembrane, and a short cytoplasmic stretch. In the developing nervous system, CALEB is associated with glial and neuronal surfaces. CALEB is composed of a 140/130-kD doublet, an 80-kD band, and a chondroitinsulfate-containing 200-kD component. The latter two components are expressed in the embryonic nervous system and are downregulated in the adult nervous system. CALEB binds to the extracellular matrix glycoproteins tenascin-C and -R. In vitro antibody perturbation experiments reveal a participation of CALEB in neurite formation in a permissive environment.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Blocking/pharmacology Avian Proteins Axons/metabolism Brain Chemistry Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal/metabolism Cell Membrane/metabolism Chick Embryo Chickens Chondroitin Sulfates/chemistry Epidermal Growth Factor/immunology,metabolism,physiology Leucine Membrane Glycoproteins/immunology,metabolism,physiology Molecular Sequence Data Nerve Tissue Proteins/immunology,metabolism,physiology Neurites/immunology,physiology Neuroglia/metabolism Neurons/metabolism Receptors, Antigen/metabolism Retina/metabolism Substrate Specificity Tenascin/metabolism
Chemicals
Antibodies, Blocking Avian Proteins CALEB protein, chicken Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal Membrane Glycoproteins NRCAM protein, Gallus gallus Nerve Tissue Proteins Receptors, Antigen Tenascin Tn receptor Epidermal Growth Factor Chondroitin Sulfates Leucine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schumacher S
Max-Delbruck-Centrum fur Molekulare Medizin, Berlin, Germany.
Volkmer H
Buck F
Otto A
Tarnók A
Roth S
Rathjen F G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.136.4.895
Published
1997-02-24
Pages
895-906
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132487
Subset
IM
Databases
GENBANK
Y09264
External Links
PubMed source DOI: 10.1083/jcb.136.4.895 PMC Full Text View journal details
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