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PMID: 9053457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin.

The Journal of experimental medicine ·Vol. 185 ·No. 3 ·1997-02-03 ·Pages 573-8

Borges E, Tietz W, Steegmaier M, Moll T, Hallmann R, Hamann A, Vestweber D

Abstract

We have shown recently that mouse Th1 cells but not Th2 cells are selectively recruited into inflamed sites of a delayed-type hypersensitivity (DTH) reaction of the skin. This migration was blocked by monoclonal antibodies (mAb) against P- and E-selectin. Here we show that Th1 cells bind to P-selectin via the P-selectin glycoprotein ligand-1 (PSGL-1). This is the only glycoprotein ligand that was detectable by affinity isolation with a P-selectin-Ig fusion protein. Binding of Th1 cells to P-selectin, as analyzed by flow cytometry and in cell adhesion assays, was completely blocked by antibodies against PSGL-1. The same antibodies blocked partially the migration of Th1 cells into cutaneous DTH reactions. This blocking activity, in combination with that of a mAb against E-selectin, was additive. PSGL-1 on Th2 cells, although expressed at similar levels as on Th1 cells, did not support binding to P-selectin. Thus, the P-selectin-binding form of PSGL-1 distinguishes Th1 cells from Th2 cells. Furthermore, PSGL-1 is relevant for the entry of Th1 cells into inflamed areas of the skin. This is the first demonstration for the importance of PSGL-1 for mouse leukocyte recruitment in vivo.

MeSH Terms
Animals Cell Movement Cells, Cultured Dermatitis/immunology Membrane Glycoproteins/physiology Mice Mice, Inbred BALB C P-Selectin/metabolism Th1 Cells/physiology Th2 Cells/physiology
Chemicals
Membrane Glycoproteins P-Selectin P-selectin ligand protein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Borges E
Institut für Zellbiologie, ZMBE, Universität Münster, Germany.
Tietz W
Steegmaier M
Moll T
Hallmann R
Hamann A
Vestweber D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.185.3.573
Published
1997-02-03
Pages
573-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2196023
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.185.3.573 PMC Full Text View journal details
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