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PMID: 9085570 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment.

Plant physiology ·Vol. 113 ·No. 3 ·1997-03-00 ·Pages 729-38

Bell-Lelong DA, Cusumano JC, Meyer K, Chapple C

Abstract

Cinnamate-4-hydroxylase (C4H) is the first Cyt P450-dependent monooxygenase of the phenylpropanoid pathway. To study the expression of this gene in Arabidopsis thaliana, a C4H cDNA clone from the Arabidopsis expressed sequence tag database was identified and used to isolate its corresponding genomic clone. The entire C4H coding sequence plus 2.9 kb of its promoter were isolated on a 5.4-kb HindIII fragment of this cosmid. Inspection of the promoter sequence revealed the presence of a number of putative regulatory motifs previously identified in the promoters of other phenylpropanoid pathway genes. The expression of C4H was analyzed by RNA blot hybridization analysis and in transgenic Arabidopsis carrying a C4H-beta-glucuronidase transcriptional fusion. C4H message accumulation was light-dependent, but was detectable even in dark-grown seedlings. Consistent with these data, C4H mRNA was accumulated to light-grown levels in etiolated det1-1 mutant seedlings. C4H is widely expressed in various Arabidopsis tissues, particularly in roots and cells undergoing lignification. The C4H-driven beta-glucuronidase expression accurately reflected the tissue-specificity and wound-inducibility of the C4H promoter indicated by RNA blot hybridization analysis. A modest increase in C4H expression was observed in the tt8 mutant of Arabidopsis.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics,growth & development Arabidopsis Proteins Base Sequence Cloning, Molecular Cytochrome P-450 Enzyme System/genetics DNA, Complementary Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glucuronidase/genetics Mixed Function Oxygenases/genetics Molecular Sequence Data Plants, Genetically Modified Trans-Cinnamate 4-Monooxygenase
Chemicals
Arabidopsis Proteins CYP73A5 protein, Arabidopsis DNA, Complementary Cytochrome P-450 Enzyme System Mixed Function Oxygenases Trans-Cinnamate 4-Monooxygenase Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bell-Lelong D A
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Cusumano J C
Meyer K
Chapple C
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.113.3.729
Published
1997-03-00
Pages
729-38
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC158190
Subset
IM
Databases
GENBANK
U71080, U71081
External Links
PubMed source DOI: 10.1104/pp.113.3.729 PMC Full Text View journal details
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