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PMID: 9089438 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transfer of voltage independence from a rat olfactory channel to the Drosophila ether-à-go-go K+ channel.

The Journal of general physiology ·Vol. 109 ·No. 3 ·1997-03-00 ·Pages 301-11

Tang CY, Papazian DM

Abstract

The S4 segment is an important part of the voltage sensor in voltage-gated ion channels. Cyclic nucleotide-gated channels, which are members of the superfamily of voltage-gated channels, have little inherent sensitivity to voltage despite the presence of an S4 segment. We made chimeras between a voltage-independent rat olfactory channel (rolf) and the voltage-dependent ether-à-go-go K+ channel (eag) to determine the basis of their divergent gating properties. We found that the rolf S4 segment can support a voltage-dependent mechanism of activation in eag, suggesting that rolf has a potentially functional voltage sensor that is silent during gating. In addition, we found that the S3-S4 loop of rolf increases the relative stability of the open conformation of eag, effectively converting eag into a voltage-independent channel. A single charged residue in the loop makes a significant contribution to the relative stabilization of the open stage in eag. Our data suggest that cyclic nucleotide-gated channels such as rolf contain a voltage sensor which, in the physiological voltage range, is stabilized in an activated conformation that is permissive for pore opening.

MeSH Terms
Amino Acid Sequence Animals Biotransformation/physiology DNA, Complementary/biosynthesis Drosophila Drosophila Proteins Electrophysiology Ether-A-Go-Go Potassium Channels Ion Channel Gating/physiology Membrane Potentials/physiology Molecular Sequence Data Mutation/physiology Patch-Clamp Techniques Potassium Channels/genetics,physiology Rats Recombinant Fusion Proteins/genetics,metabolism Smell/physiology
Chemicals
DNA, Complementary Drosophila Proteins Ether-A-Go-Go Potassium Channels Potassium Channels Recombinant Fusion Proteins eag protein, Drosophila
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tang C Y
Department of Physiology, School of Medicine, University of California, Los Angeles 90095-1751, USA.
Papazian D M
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46 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.109.3.301
Published
1997-03-00
Pages
301-11
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2217070
Subset
IM
Grants
NIGMS NIH HHS · R01 GM043459-15 · United States
NIGMS NIH HHS · GM43459 · United States
NIGMS NIH HHS · R01 GM043459-11 · United States
NIGMS NIH HHS · R01 GM043459-16 · United States
NIGMS NIH HHS · R01 GM043459-10 · United States
NIGMS NIH HHS · R01 GM043459 · United States
NIGMS NIH HHS · R01 GM043459-13 · United States
NIGMS NIH HHS · R01 GM043459-17 · United States
NIGMS NIH HHS · R01 GM043459-14 · United States
NIGMS NIH HHS · R01 GM043459-12 · United States
NIGMS NIH HHS · R01 GM043459-15S1 · United States
NIGMS NIH HHS · R01 GM043459-09 · United States
External Links
PubMed source DOI: 10.1085/jgp.109.3.301 PMC Full Text View journal details
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