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PMID: 9094991 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tenascin-C, proliferation and subendothelial fibronectin in progressive pulmonary vascular disease.

The American journal of pathology ·Vol. 150 ·No. 4 ·1997-04-00 ·Pages 1349-60

Jones PL, Cowan KN, Rabinovitch M

Abstract

Progressive pulmonary hypertension is characterized by smooth muscle cell proliferation and migration leading to occlusive arterial lesions. Previously, using cultured smooth muscle cells, we demonstrated that epidermal growth factor (EGF)-dependent proliferation and migration are dependent on tenascin-C (Tn) and cellular fibronectin (Fn), respectively. In this study we applied immunohistochemistry to lung biopsy tissue from patients with congenital heart defects and pulmonary hypertension to determine how the distribution and intensity of Tn, EGF, proliferating cell nuclear antigen (PCNA), and Fn expression related to arterial abnormalities. With mildly increased wall thickness, minimal Tn, PCNA, and EGF was evident. With progressive hypertrophy, moderately intense foci of Tn were apparent in the adventitia, periendothelium, and occasionally the media but not consistently co-distributing with EGF and PCNA. With obstructive lesions, intense neointimal Tn expression co-localized with EGF and PCNA. Fn accumulation in the periendothelium increased with medial hypertrophy and became more widespread in a diffuse pattern with neointimal formation. The neointima was predominantly composed of alpha-smooth-muscle-actin-positive cells, occasional inflammatory cells with no evidence of apoptosis. These studies are consistent with Tn modulating EGF-dependent neointimal smooth muscle cell proliferation and Fn providing a gradient for smooth muscle cell migration from media to neointima.

MeSH Terms
Adolescent Apoptosis Cell Division Child Child, Preschool Endothelium, Vascular/metabolism,pathology Epidermal Growth Factor/analysis Female Fibronectins/analysis Heart Defects, Congenital/metabolism,pathology Humans Hypertension, Pulmonary/metabolism,pathology Infant Male Proliferating Cell Nuclear Antigen/analysis Tenascin/analysis Tunica Intima/metabolism,pathology Tunica Media/metabolism,pathology
Chemicals
Fibronectins Proliferating Cell Nuclear Antigen Tenascin Epidermal Growth Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jones P L
Division of Cardiovascular Research, Hospital for Sick Children, Toronto, Ontario, Canada.
Cowan K N
Rabinovitch M
References (58)
58 references, click to expand
  1. Lung biopsy in congenital heart disease: a morphometric approach to pulmonary vascular disease.
    Circulation. 1978 Dec;58(6):1107-22 PMID: 709766
  2. Suppression of apoptosis by basement membrane requires three-dimensional tissue organization and withdrawal from the cell cycle.
    Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3509-13 PMID: 8622967
  3. Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis.
    Cell. 1986 Oct 10;47(1):131-9 PMID: 2428505
  4. Hexabrachion proteins in embryonic chicken tissues and human tumors.
    J Cell Biol. 1987 Sep;105(3):1387-94 PMID: 3654758
  5. Altered elastin and collagen synthesis associated with progressive pulmonary hypertension induced by monocrotaline. A biochemical and ultrastructural study.
    Lab Invest. 1988 Feb;58(2):184-95 PMID: 3123799
  6. Induction of tenascin in healing wounds.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2757-67 PMID: 2462568
  7. Regional heterogeneity of elastin and collagen gene expression in intralobar arteries in response to hypoxic pulmonary hypertension as demonstrated by in situ hybridization.
    Am J Pathol. 1989 Dec;135(6):1073-88 PMID: 2596571
  8. Stimulation of tenascin expression in mesenchyme by epithelial-mesenchymal interactions.
    Int J Dev Biol. 1989 Mar;33(1):71-9 PMID: 2484680
  9. Fibronectin, hyaluronan, and a hyaluronan binding protein contribute to increased ductus arteriosus smooth muscle cell migration.
    Dev Biol. 1991 Feb;143(2):235-47 PMID: 1703972
  10. Developmentally regulated changes in extracellular matrix in endothelial and smooth muscle cells in the ductus arteriosus may be related to intimal proliferation.
    Lab Invest. 1991 Feb;64(2):187-99 PMID: 1997732
  11. Proliferation of smooth muscle cells in the inner and outer layers of the tunica media of arteries: an in vitro study.
    J Cell Physiol. 1991 Jun;147(3):385-95 PMID: 2066360
  12. Induction of tenascin in rat arterial injury. Relationship to altered smooth muscle cell phenotype.
    Am J Pathol. 1991 Sep;139(3):649-56 PMID: 1716048
  13. Phenotypic changes of human aortic smooth muscle cells during development and in the adult vessel.
    Am J Physiol. 1991 Oct;261(4 Suppl):78-80 PMID: 1928456
  14. Chronic hypoxic pulmonary hypertension in rats and increased elastolytic activity.
    Am J Physiol. 1991 Dec;261(6 Pt 2):H1716-26 PMID: 1836309
  15. Extracellular matrix protein gene expression in atherosclerotic hypertensive pulmonary arteries.
    Am J Pathol. 1992 Feb;140(2):357-64 PMID: 1739129
  16. Functional interplay between interleukin-1 receptor and elastin binding protein regulates fibronectin production in coronary artery smooth muscle cells.
    Exp Cell Res. 1996 May 25;225(1):122-31 PMID: 8635505
  17. Elafin, a serine elastase inhibitor, attenuates post-cardiac transplant coronary arteriopathy and reduces myocardial necrosis in rabbits afer heterotopic cardiac transplantation.
    J Clin Invest. 1996 Jun 1;97(11):2452-68 PMID: 8647937
  18. The role of vascular injury and hemodynamics in rat pulmonary artery remodeling.
    J Clin Invest. 1996 Jul 15;98(2):434-42 PMID: 8755654
  19. Tenascin-C is induced with progressive pulmonary vascular disease in rats and is functionally related to increased smooth muscle cell proliferation.
    Circ Res. 1996 Dec;79(6):1131-42 PMID: 8943951
  20. The pathology of hypertensive pulmonary vascular disease; a description of six grades of structural changes in the pulmonary arteries with special reference to congenital cardiac septal defects.
    Circulation. 1958 Oct;18(4 Part 1):533-47 PMID: 13573570
  21. The extracellular matrix of lip wounds in fetal, neonatal and adult mice.
    Development. 1991 Jun;112(2):651-68 PMID: 1724421
  22. Expression of tenascin in the developing and adult cerebellar cortex.
    J Neurosci. 1992 Mar;12(3):736-49 PMID: 1372043
  23. Apoptotic cell death and tissue remodelling during mouse mammary gland involution.
    Development. 1992 May;115(1):49-58 PMID: 1638991
  24. Expression of tenascin by vascular smooth muscle cells. Alterations in hypertensive rats and stimulation by angiotensin II.
    Am J Pathol. 1992 Aug;141(2):377-88 PMID: 1379781
  25. Angiotensin II regulates tenascin gene expression in vascular smooth muscle cells.
    J Biol Chem. 1992 Nov 25;267(33):23910-5 PMID: 1385427
  26. Neointimal macrophages colocalize with extracellular matrix gene expression in human atherosclerotic pulmonary arteries.
    J Clin Invest. 1993 Feb;91(2):588-94 PMID: 8432864
  27. Tenascin expression in the mouse: in situ localization and induction in vitro by bFGF.
    J Cell Sci. 1993 Jan;104 ( Pt 1):69-76 PMID: 7680659
  28. Increased interleukin-1 beta and fibronectin expression are early features of the development of the postcardiac transplant coronary arteriopathy in piglets.
    Am J Pathol. 1993 Jun;142(6):1772-86 PMID: 8506947
  29. Active collagen synthesis by pulmonary arteries in human primary pulmonary hypertension.
    Am J Pathol. 1993 Jul;143(1):121-9 PMID: 7686340
  30. Endothelial cell attachment and spreading on human tenascin is mediated by alpha 2 beta 1 and alpha v beta 3 integrins.
    J Cell Sci. 1993 Aug;105 ( Pt 4):1001-12 PMID: 7693733
  31. Regulation of astrocytic tenascin by basic fibroblast growth factor.
    Dev Biol. 1993 Dec;160(2):480-93 PMID: 7504635
  32. Regional differences in the distribution of the proteoglycans biglycan and decorin in the extracellular matrix of atherosclerotic and restenotic human coronary arteries.
    Am J Pathol. 1994 May;144(5):962-74 PMID: 8178945
  33. In vivo blockade of tumor necrosis factor-alpha in cholesterol-fed rabbits after cardiac transplant inhibits acute coronary artery neointimal formation.
    Circulation. 1994 Jun;89(6):2768-79 PMID: 8205691
  34. Induction of human tenascin (neuronectin) by growth factors and cytokines: cell type-specific signals and signalling pathways.
    J Cell Sci. 1994 Feb;107 ( Pt 2):487-97 PMID: 7515896
  35. Post-cardiac transplant arteriopathy in piglets is associated with fragmentation of elastin and increased activity of a serine elastase.
    Am J Pathol. 1994 Jul;145(1):202-10 PMID: 8030749
  36. The extracellular matrix ligands fibronectin and tenascin collaborate in regulating collagenase gene expression in fibroblasts.
    Mol Biol Cell. 1994 Apr;5(4):439-53 PMID: 7519905
  37. Smooth muscle cell migration and matrix metalloproteinase expression after arterial injury in the rat.
    Circ Res. 1994 Sep;75(3):539-45 PMID: 8062427
  38. The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin.
    J Clin Invest. 1994 Sep;94(3):1163-71 PMID: 8083356
  39. Persistence, re-expression, and induction of pulmonary arterial fibronectin, tropoelastin, and type I procollagen mRNA expression in neonatal hypoxic pulmonary hypertension.
    Am J Pathol. 1994 Dec;145(6):1411-20 PMID: 7992844
  40. Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels.
    Cell. 1994 Dec 30;79(7):1157-64 PMID: 7528107
  41. Tenascin-C expression by fibroblasts is elevated in stressed collagen gels.
    J Cell Biol. 1994 Dec;127(6 Pt 2):2093-101 PMID: 7528751
  42. Apoptosis (programmed cell death) in arteries of the neonatal lamb.
    Circ Res. 1995 Feb;76(2):168-75 PMID: 7834826
  43. Heterogeneity in migration of smooth muscle cells from normal and injured rat thoracic aorta in primary culture.
    Cardiovasc Res. 1995 Jan;29(1):38-43 PMID: 7895237
  44. Identification of 92-kD gelatinase in human coronary atherosclerotic lesions. Association of active enzyme synthesis with unstable angina.
    Circulation. 1995 Apr 15;91(8):2125-31 PMID: 7697840
  45. Apoptosis participates in cellularity regulation during rat aortic intimal thickening.
    Am J Pathol. 1995 May;146(5):1059-64 PMID: 7747800
  46. Cytokines in human renal interstitial fibrosis. II. Intrinsic interleukin (IL)-1 synthesis and IL-1-dependent production of IL-6 and IL-8 by cultured kidney fibroblasts.
    Kidney Int. 1995 Mar;47(3):845-54 PMID: 7752583
  47. Tenascin-C inhibits extracellular matrix-dependent gene expression in mammary epithelial cells. Localization of active regions using recombinant tenascin fragments.
    J Cell Sci. 1995 Feb;108 ( Pt 2):519-27 PMID: 7539436
  48. Blockade of very late antigen-4 integrin binding to fibronectin with connecting segment-1 peptide reduces accelerated coronary arteriopathy in rabbit cardiac allografts.
    J Clin Invest. 1995 Jun;95(6):2601-10 PMID: 7539456
  49. Functional aspects of vascular tenascin-C expression.
    J Vasc Res. 1995 May-Jun;32(3):162-74 PMID: 7539634
  50. The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression.
    Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6161-5 PMID: 7541142
  51. Hypoxia selectively induces proliferation in a specific subpopulation of smooth muscle cells in the bovine neonatal pulmonary arterial media.
    J Clin Invest. 1995 Jul;96(1):273-81 PMID: 7615796
  52. Death by any other name.
    Am J Pathol. 1995 Aug;147(2):229-34 PMID: 7639321
  53. Evidence for apoptosis in advanced human atheroma. Colocalization with interleukin-1 beta-converting enzyme.
    Am J Pathol. 1995 Aug;147(2):251-66 PMID: 7639325
  54. Evidence for apoptosis in human atherogenesis and in a rat vascular injury model.
    Am J Pathol. 1995 Aug;147(2):267-77 PMID: 7639326
  55. Interstitial collagenase (MMP-1) expression in human carotid atherosclerosis.
    Circulation. 1995 Sep 15;92(6):1393-8 PMID: 7664418
  56. Tenascin: a potential role in human arteriovenous PTFE graft failure.
    J Surg Res. 1996 Feb 1;60(2):409-16 PMID: 8598678
  57. Exogenous leukocyte and endogenous elastases can mediate mitogenic activity in pulmonary artery smooth muscle cells by release of extracellular-matrix bound basic fibroblast growth factor.
    J Cell Physiol. 1996 Mar;166(3):495-505 PMID: 8600153
  58. Chick myotendinous antigen. II. A novel extracellular glycoprotein complex consisting of large disulfide-linked subunits.
    J Cell Biol. 1984 Jun;98(6):1937-46 PMID: 6202699
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1997-04-00
Pages
1349-60
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1858188
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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