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PMID: 9097040 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.

DNA research : an international journal for rapid publication of reports on genes and genomes ·Vol. 3 ·No. 6 ·1996-12-31 ·Pages 379-92

Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T

Abstract

The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.

MeSH Terms

Attachment Sites, Microbiological Chromosome Mapping DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial/genetics Multigene Family Open Reading Frames RNA, Transfer, Asn/genetics Replicon Sequence Analysis, DNA Sequence Homology, Amino Acid

Chemicals

DNA, Bacterial RNA, Transfer, Asn

Authors & Affiliations

25 authors, click to expand affiliations / ORCID

Itoh T

Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Japan.

Aiba H

Baba T

Hayashi K

Inada T

Isono K

Kasai H

Kimura S

Kitakawa M

Kitagawa M

Makino K

Miki T

Mizobuchi K

Mori H

Mori T

Motomura K

Nakade S

Nakamura Y

Nashimoto H

Nishio Y

Oshima T

Saito N

Sampei G

Seki Y

Horiuchi T

Article Info

Journal

DNA research : an international journal for rapid publication of reports on genes and genomes

Abbr.

DNA Res

ISSN

1340-2838

DOI

10.1093/dnares/3.6.379

Published

1996-12-31

Pages

379-92

Language

English

Region

England

NLM ID

9423827

Subset

Databases

GENBANK

D90822, D90824, D90825, D90826, D90827, D90828, D90829, D90830, D90831, D90832, D90833, D90834, D90835, D90836, D90837, D90838, D90839, D90840, D90841, D90842, D90843, D90844, D90845, D90846, D90847, D90848, D90849, D90850, D90851

External Links

PubMed source DOI: 10.1093/dnares/3.6.379 View journal details

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A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.

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