Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes.

DNA Primers DNA Restriction Enzymes/genetics,metabolism DNA-Directed DNA Polymerase/metabolism Electrophoresis, Agar Gel Fluorescein-5-isothiocyanate/analogs & derivatives Fluorescent Dyes/metabolism Gelatinases/genetics Matrix Metalloproteinase 2 Metalloendopeptidases/genetics Mutagenesis, Site-Directed Nucleic Acid Hybridization Plasmids/genetics Polymerase Chain Reaction/methods Taq Polymerase

DNA Primers Fluorescent Dyes Taq Polymerase DNA-Directed DNA Polymerase DNA Restriction Enzymes Gelatinases Metalloendopeptidases Matrix Metalloproteinase 2 Fluorescein-5-isothiocyanate