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PMID: 9106518 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment.

Plant molecular biology ·Vol. 33 ·No. 5 ·1997-03-00 ·Pages 943-6

van der Fits L, Memelink J

Abstract

Activities of several CaMV 35S and FMV 34S promoter derivatives fused to the gusA reporter gene were compared in suspension-cultured Catharanthus roseus cells that were transiently and stably transformed using particle bombardment. Our data demonstrate that the 35S and a deletion derivative of the 34S promoter combined with particle bombardment form useful tools for genetic engineering of C. roseus cells. Our results disagree on several points with activities of 35S and 34S promoter derivatives reported for tobacco, indicating that absolute and relative promoter activities can differ between plant species.

MeSH Terms
Alkaloids/biosynthesis Caulimovirus/genetics Cells, Cultured Genes, Reporter Genetic Engineering/methods Genetic Techniques Glucuronidase/genetics Mosaic Viruses/genetics Plants, Medicinal/genetics Promoter Regions, Genetic/genetics Transformation, Genetic
Chemicals
Alkaloids Glucuronidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van der Fits L
Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, the Netherlands.
Memelink J
References (13)
13 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
Published
1997-03-00
Pages
943-6
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
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