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PMID: 9108050 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Caveolin mRNA levels are up-regulated by free cholesterol and down-regulated by oxysterols in fibroblast monolayers.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 8 ·1997-04-15 ·Pages 3753-8

Fielding CJ, Bist A, Fielding PE

Abstract

In confluent fibroblast monolayers, an increase in the selective uptake of free cholesterol (FC) from plasma low density lipoprotein (LDL) was accompanied by an increase in FC efflux. The rate of FC efflux was proportional to the FC content of the cell surface caveolae and to mRNA levels of caveolin, an FC-binding protein of caveolae. Inhibitors of LDL-FC internalization reduced the increase in caveolin mRNA levels and FC efflux. Oxysterols reduced caveolin mRNA levels, as well as transport of FC to the cell surface and FC efflux. DNA antisense to caveolin reduced caveolin mRNA and inhibited FC efflux. These data suggest that regulation of FC efflux can contribute to cellular FC homeostasis when LDL levels are modified over the physiological range, and they link this regulation to caveolin.

MeSH Terms
Biological Transport Caveolin 1 Caveolins Cells, Cultured Cholesterol/metabolism,pharmacology Down-Regulation/drug effects Fibroblasts/metabolism Humans Lipoproteins, LDL/metabolism Membrane Proteins/genetics,metabolism Oxidation-Reduction RNA, Messenger/genetics,metabolism Up-Regulation/drug effects
Chemicals
CAV1 protein, human Caveolin 1 Caveolins Lipoproteins, LDL Membrane Proteins RNA, Messenger Cholesterol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fielding C J
Cardiovascular Research Institute, Department of Physiology, University of California Medical Center, San Francisco 94143, USA.
Bist A
Fielding P E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.8.3753
Published
1997-04-15
Pages
3753-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20513
Subset
IM
Grants
NHLBI NIH HHS · HL 14237 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.8.3753 PMC Full Text View journal details
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