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PMID: 9119082 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Desensitization of the P2X(2) receptor controlled by alternative splicing.

FEBS letters ·Vol. 404 ·No. 2-3 ·1997-03-10 ·Pages 294-8

Brändle U, Spielmanns P, Osteroth R, Sim J, Surprenant A, Buell G, Ruppersberg JP, Plinkert PK, Zenner HP, Glowatzki E

Abstract

P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X(2) receptor splice variant (P2X(2-2)) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X(2) gene. P2X(2-2) is as highly expressed as the original P2X(2) sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X(2) or P2X(2-2) receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X(2) isoforms.

MeSH Terms

Adenosine Triphosphate/pharmacology Alternative Splicing Amino Acid Sequence Animals Base Sequence Brain/metabolism Cell Line Female Genetic Variation Humans Intestinal Mucosa/metabolism Kidney/metabolism Membrane Potentials/drug effects Molecular Sequence Data Mutagenesis, Site-Directed Oocytes/physiology Organ Specificity Organ of Corti/metabolism Patch-Clamp Techniques Rats Rats, Wistar Receptors, Purinergic P2/biosynthesis,chemistry,physiology Receptors, Purinergic P2X2 Recombinant Proteins/biosynthesis,chemistry,metabolism Sequence Deletion Spleen/metabolism Transfection Xenopus laevis

Chemicals

P2RX2 protein, human Receptors, Purinergic P2 Receptors, Purinergic P2X2 Recombinant Proteins Adenosine Triphosphate

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Brändle U

Department of Otolaryngology, University of Tübingen, Germany.

Spielmanns P

Osteroth R

Sim J

Surprenant A

Buell G

Ruppersberg J P

Plinkert P K

Zenner H P

Glowatzki E

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/s0014-5793(97)00128-2

Published

1997-03-10

Pages

294-8

Language

English

Region

England

NLM ID

0155157

Subset

Databases

GENBANK

Y09910

External Links

PubMed source DOI: 10.1016/s0014-5793(97)00128-2 View journal details

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Desensitization of the P2X(2) receptor controlled by alternative splicing.

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