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PMID: 9122232 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA methylation and genetic instability in colorectal cancer cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 6 ·1997-03-18 ·Pages 2545-50

Lengauer C, Kinzler KW, Vogelstein B

Abstract

Apparent alterations in DNA methylation have been observed in many cancers, but whether such alterations represent a persistent alteration in the normal methylation process is not known. In this study, we report a striking difference in the expression of exogenously introduced retroviral genes in various colorectal cancer cell lines. Extinguished expression was associated with DNA methylation and could be reversed by treatment with the demethylating agent 5-azacytidine. A striking correlation between genetic instability and methylation capacity suggested that methylation abnormalities may play a role in chromosome segregation processes in cancer cells.

MeSH Terms
Azacitidine/pharmacology Base Sequence Cell Line Colorectal Neoplasms/genetics DNA Methylation DNA, Neoplasm/chemistry,drug effects,metabolism Genes, Viral Humans Kanamycin Kinase Molecular Sequence Data Phosphotransferases (Alcohol Group Acceptor)/biosynthesis Polymerase Chain Reaction Protein Biosynthesis Recombinant Proteins/biosynthesis Repetitive Sequences, Nucleic Acid Retroviridae/genetics Transcription, Genetic Transfection Tumor Cells, Cultured beta-Galactosidase/biosynthesis
Chemicals
DNA, Neoplasm Recombinant Proteins Phosphotransferases (Alcohol Group Acceptor) Kanamycin Kinase beta-Galactosidase Azacitidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lengauer C
Howard Hughes Medical Institute at The Johns Hopkins Oncology Center, Baltimore, MD 21231, USA.
Kinzler K W
Vogelstein B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.6.2545
Published
1997-03-18
Pages
2545-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20125
Subset
IM
Grants
NCI NIH HHS · P50 CA062924 · United States
NCI NIH HHS · CA 35494 · United States
NCI NIH HHS · CA 62924 · United States
Corrections
CommentIn
PMID: 9122155
External Links
PubMed source DOI: 10.1073/pnas.94.6.2545 PMC Full Text View journal details
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