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PMID: 9155569 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of N alpha-methyl-histamine on acid secretion in isolated cultured rabbit parietal cells: implications for Helicobacter pylori associated gastritis and gastric physiology.

Gut ·Vol. 40 ·No. 1 ·1997-01-00 ·Pages 14-9

Beales IL, Calam J

Abstract

Helicobacter pylori has been shown to produce the unusual metabolite N alpha-methyl-histamine. This compound is known to be a potent agonist at inhibitory histamine H3 receptors. There is increasing evidence implicating this receptor in the control of gastric acid secretion but the mechanism for this remains to be clarified. To investigate the effect of N alpha-methyl-histamine on the acid secretory activity of parietal cells and to determine the mechanism for such effects, thus helping to determine the role of this compound in the pathophysiology of H pylori infection. Rabbit parietal cells were isolated and enriched by collagenase-EDTA digestion and centrifugal elutriation. Following culture on Matrigel coated plates, acid secretion was assessed by 14C aminopyrine accumulation. N alpha-methyl-histamine (100 microM) was as potent as histamine (100 microM) in stimulating acid secretion. This effect was reversed by ranitidine indicating it was mediated via the H2 receptor. N alpha-methyl-histamine potentiated the effects of both carbachol (increased by 280%) and gastrin (by 350%) (p < 0.01). N alpha-methyl-histamine had no inhibitory actions on forskolin or carbachol stimulated acid secretion suggesting that there is not an inhibitory H3 receptor located directly on the parietal cell. Bacterially produced N alpha-methyl-histamine directly stimulates acid secretion by parietal cells and this may contribute to the increased acid secretion that contributes to duodenal ulceration.

MeSH Terms
Aminopyrine/analysis Animals Carbachol/pharmacology Carbon Radioisotopes/analysis Cells, Cultured Colforsin/pharmacology Gastric Acid/metabolism Histamine H2 Antagonists/pharmacology Methylhistamines/pharmacology Parasympathomimetics/pharmacology Parietal Cells, Gastric/drug effects,metabolism Rabbits Ranitidine/pharmacology Receptors, Histamine H2/drug effects
Chemicals
Carbon Radioisotopes Histamine H2 Antagonists Methylhistamines Parasympathomimetics Receptors, Histamine H2 Aminopyrine Colforsin Ranitidine Carbachol N-methylhistamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beales I L
Department of Gastroenterology, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Calam J
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Article Info
Journal
Gut
Abbr.
Gut
ISSN
0017-5749
DOI
10.1136/gut.40.1.14
Published
1997-01-00
Pages
14-9
Language
English
Region
England
NLM ID
2985108R
PMCID
PMC1027001
Subset
IM
External Links
PubMed source DOI: 10.1136/gut.40.1.14 PMC Full Text View journal details
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