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PMID: 9172753 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Diacylglycerol and the promotion of lamellar-hexagonal and lamellar-isotropic phase transitions in lipids: implications for membrane fusion.

Biophysical journal ·Vol. 70 ·No. 5 ·1996-05-00 ·Pages 2299-306

Basanez G, Nieva JL, Rivas E, Alonso A, Goni FM

Abstract

Changes in steady-state fluorescence anisotropy of 1 -(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene TMA-DPH) are applied to the detection of lamellar-hexagonal transitions in egg phosphatidylethanolamine. Even low (2 mole%) proportions of diacylglycerol decrease the hexagonal transition temperature considerably, as confirmed by differential scanning calorimetry. Diacylglycerol is also found to promote a lamellar to "isotropic" (Q(224) cubic) transition in mixtures of phosphatidylcholine: phosphatidylethanolamine:cholesterol. This nonreversible transition is also observed by (31)P nuclear magnetic resonance and detected as a large increase in TMA-DPH steady-state anisotropy. The same technique reveals as well that lysophosphatidylcholine counteracts the effect of diacylglycerol and stabilizes the lamellar phase in both transitions. Diacylglycerol and lysophosphatidylcholine are known to respectively promote and inhibit membrane fusion in a variety of systems. These data are interpreted in support of the hypothesis of a highly bent structural fusion intermediate ("stalk"). They also show the interest of lipid-phase studies in predicting and rationalizing membrane fusion mechanisms.

MeSH Terms
Calorimetry, Differential Scanning Cholesterol/chemistry Diglycerides/chemistry Diphenylhexatriene/analogs & derivatives Fluorescence Polarization Fluorescent Dyes Membrane Fusion Models, Biological Molecular Conformation Phosphatidylcholines/chemistry Phosphatidylethanolamines/chemistry Thermodynamics
Chemicals
Diglycerides Fluorescent Dyes Phosphatidylcholines Phosphatidylethanolamines Diphenylhexatriene 1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene Cholesterol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Basanez G
Unidad Asociada al C.S.I.C., Departamento de Bioquimica, Universidad del Pais Vasco, Bilbao, Spain.
Nieva J L
Rivas E
Alonso A
Goni F M
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(96)79795-3
Published
1996-05-00
Pages
2299-306
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1225204
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(96)79795-3 PMC Full Text View journal details
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