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PMID: 9177324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana.

Plant molecular biology ·Vol. 34 ·No. 1 ·1997-05-00 ·Pages 175-80

Hirayama T, Mitsukawa N, Shibata D, Shinozaki K

Abstract

A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) from the higher plant Arabidopsis thaliana was cloned and characterized. The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predicted AtPLC2 protein is similar to those of plant PI-PLCs and mammalian delta-type PI-PLCs. Northern blot analysis revealed that AtPLC2 is expressed constitutively whereas AtPLC1S, another gene for PI-PLC of Arabidopsis, is induced by environmental stresses such as dehydration and salinity, indicating that the function of AtPLC2 is distinct from that of AtPLC1S. The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.

MeSH Terms

Amino Acid Sequence Arabidopsis/enzymology,genetics Base Sequence Cloning, Molecular DNA, Complementary/isolation & purification Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases/biosynthesis,genetics,isolation & purification Plant Proteins/biosynthesis,genetics,isolation & purification

Chemicals

DNA, Complementary Plant Proteins Phosphoric Diester Hydrolases Phosphoinositide Phospholipase C Phosphatidylinositol Diacylglycerol-Lyase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hirayama T

Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Ibaraki, Japan.

Mitsukawa N

Shibata D

Shinozaki K

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14 references, click to expand

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1023/a:1005885230896

Published

1997-05-00

Pages

175-80

Language

English

Region

Netherlands

NLM ID

9106343

Subset

Databases

GENBANK

D50804

External Links

PubMed source DOI: 10.1023/a:1005885230896 View journal details

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AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana.

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