Abstract
A methodology is presented to culture Fall Armyworm Ovary cells in simulated micrograviy using a novel bioreactor developed by NASA, the High-Aspect Ratio Vessel. In this vessel, the growth and metabolic profile for these insect cells were profoundly different than those obtained in shaker-flask culture. Specifically, stationary phase in the NASA vessel was extended from 24 h to at least 7 d while cell concentration and viability remained in excess of 1 x 10(7) viable cells/ml and 90%, respectively. Measurements of glucose utilization, lactate production, ammonia production, and pH change indicate that simulated microgravity had a twofold effect on cell metabolism. Fewer nutrients were consumed and fewer wastes were produced in stationary phase by as much as a factor of 4 over that achieved in shaker culture. Those nutrients that were consumed in the NASA vessel were directed along different metabolic pathways as evidenced by an extreme shift in glucose utilization from consumption to production in lag phase and a decrease in yield coefficients by one half in stationary phase. These changes reflect a reduction in hydrodynamic forces from over 1 dyne/cm2 in shaker culture to under 0.5 dyne/cm2 in the NASA vessel. These results suggest that cultivation of insect cells in simulated microgravity may reduce production costs of cell-derived biologicals by extending production time and reducing medium requirements.
MeSH Terms
Ammonia/pharmacology
Animals
Cell Division
Cell Line
Cell Survival
Culture Media/pharmacology
Female
Glucose/pharmacology
Hydrogen-Ion Concentration
Lactates/pharmacology
Ovary/cytology
Space Simulation/instrumentation
Spodoptera/cytology
Weightlessness
Chemicals
Culture Media
Lactates
Ammonia
Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Francis K M
Department of Chemical Engineering, Tulane University, New Orleans, Louisiana 70118, USA.
O'Connor K C
Spaulding G F
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