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PMID: 9199813 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Barriers to diffusion of plasma membrane proteins form early during guinea pig spermiogenesis.

Biophysical journal ·Vol. 73 ·No. 1 ·1997-07-00 ·Pages 507-16

Cowan AE, Nakhimovsky L, Myles DG, Koppel DE

Abstract

The plasma membrane of the mature guinea pig sperm is segregated into at least four domains of different composition. Previous studies have shown that some proteins localized within these domains are free to diffuse laterally, suggesting that barriers to protein diffusion are responsible for maintaining the nonuniform distribution of at least some surface proteins in mature sperm. The different membrane domains appear sequentially during sperm morphogenesis in the testis and during later passage through the epididymis. To determine when diffusion barriers become functional during sperm development, we examined the diffusion of two proteins that are expressed on the cell surface of developing spermatids and become segregated to different plasma membrane domains during the course of spermiogenesis. Both proteins exhibited rapid lateral diffusion throughout spermiogenesis, even after they become localized to specific regions of the surface membrane. These results suggest that barriers to membrane diffusion form concomitantly with membrane domains during spermiogenesis.

MeSH Terms
ADAM Proteins Animals Antibodies, Monoclonal Biotin Cell Membrane/physiology Diffusion Fertilins Guinea Pigs Immunoglobulin Fab Fragments Immunoglobulin G Male Membrane Glycoproteins/analysis Membrane Proteins/analysis,physiology Metalloendopeptidases/analysis Microscopy, Fluorescence/methods Spermatids/cytology,physiology Spermatocytes/cytology,physiology Spermatogenesis/physiology Spermatozoa/cytology,physiology Testis
Chemicals
Antibodies, Monoclonal Immunoglobulin Fab Fragments Immunoglobulin G Membrane Glycoproteins Membrane Proteins Biotin ADAM Proteins Fertilins Metalloendopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cowan A E
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA. [email protected]
Nakhimovsky L
Myles D G
Koppel D E
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(97)78089-5
Email
[email protected]
Published
1997-07-00
Pages
507-16
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1180950
Subset
IM
Grants
NIGMS NIH HHS · GM 23585 · United States
NICHD NIH HHS · HD 16580 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(97)78089-5 PMC Full Text View journal details
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