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PMID: 9207092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 14 ·1997-07-08 ·Pages 7337-42

Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ

Abstract

The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.

MeSH Terms
Amino Acid Sequence Animals CHO Cells Cloning, Molecular Cricetinae Gene Expression Regulation, Enzymologic Humans JNK Mitogen-Activated Protein Kinases MAP Kinase Kinase 4 MAP Kinase Kinase 7 Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Protein Kinases/genetics,metabolism Sequence Alignment
Chemicals
Protein Kinases JNK Mitogen-Activated Protein Kinases MAP Kinase Kinase 4 MAP Kinase Kinase 7 MAP2K7 protein, human Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tournier C
Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Whitmarsh A J
Cavanagh J
Barrett T
Davis R J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.14.7337
Published
1997-07-08
Pages
7337-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23822
Subset
IM
Grants
NCI NIH HHS · R01 CA065861 · United States
NCI NIH HHS · CA65861 · United States
Databases
GENBANK
AF003199, U93030, U93031, U93032
External Links
PubMed source DOI: 10.1073/pnas.94.14.7337 PMC Full Text View journal details
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