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PMID: 9207152 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: purification, gene cloning, and trans-Golgi localization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 14 ·1997-07-08 ·Pages 7679-84

Dhugga KS, Tiwari SC, Ray PM

Abstract

We purified from pea (Pisum sativum) tissue an approximately 40 kDa reversibly glycosylated polypeptide (RGP1) that can be glycosylated by UDP-Glc, UDP-Xyl, or UDP-Gal, and isolated a cDNA encoding it, apparently derived from a single-copy gene (Rgp1). Its predicted translation product has 364 aminoacyl residues and molecular mass of 41.5 kDa. RGP1 appears to be a membrane-peripheral protein. Immunogold labeling localizes it specifically to trans-Golgi dictyosomal cisternae. Along with other evidence, this suggests that RGP1 is involved in synthesis of xyloglucan and possibly other hemicelluloses. Corn (Zea mays) contains a biochemically similar and structurally homologous RGP1, which has been thought (it now seems mistakenly) to function in starch synthesis. The expressed sequence database also reveals close homologs of pea Rgp1 in Arabidopsis and rice (Oryza sativa). Rice possesses, in addition, a distinct but homologous sequence (Rgp2). RGP1 provides a polypeptide marker for Golgi membranes that should be useful in plant membrane studies.

MeSH Terms

Amino Acid Sequence Arabidopsis Proteins Cell Wall/metabolism Cloning, Molecular Glycoproteins/genetics,isolation & purification Golgi Apparatus/metabolism Molecular Sequence Data Peas/genetics Plant Proteins/genetics,isolation & purification Sequence Alignment

Chemicals

Arabidopsis Proteins Glycoproteins Plant Proteins RGP1 protein, Arabidopsis

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dhugga K S

Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.

Tiwari S C

Ray P M

References (18)

18 references, click to expand

Reductase activity encoded by the HM1 disease resistance gene in maize.
Science. 1992 Nov 6;258(5084):985-7 PMID: 1359642
Evidence for a UDP-Glucose Transporter in Golgi Apparatus-Derived Vesicles from Pea and Its Possible Role in Polysaccharide Biosynthesis.
Plant Physiol. 1996 Dec;112(4):1585-1594 PMID: 12226465
Effect of brefeldin A on the structure of the Golgi apparatus and on the synthesis and secretion of proteins and polysaccharides in sycamore maple (Acer pseudoplatanus) suspension-cultured cells.
Plant Physiol. 1993 Apr;101(4):1363-73 PMID: 8310065
Isolation of the gene encoding yeast DNA polymerase I.
Cell. 1985 Nov;43(1):369-77 PMID: 3907855
Auxin and Fusicoccin Enhancement of beta-Glucan Synthase in Peas : An Intracellular Enzyme Activity Apparently Modulated by Proton Extrusion.
Plant Physiol. 1985 Jul;78(3):466-72 PMID: 16664267
Purification of 1,3-beta-D-glucan synthase activity from pea tissue. Two polypeptides of 55 kDa and 70 kDa copurify with enzyme activity.
Eur J Biochem. 1994 Mar 15;220(3):943-53 PMID: 8143748
Ac induces homologous recombination at the maize P locus.
Genetics. 1991 May;128(1):163-73 PMID: 1648001
A guide to naming sequenced plant genes.
Plant Mol Biol. 1996 Jan;30(2):225-7 PMID: 8616247
Functional compartmentation of the Golgi apparatus of plant cells : immunocytochemical analysis of high-pressure frozen- and freeze-substituted sycamore maple suspension culture cells.
Plant Physiol. 1992 Jul;99(3):1070-83 PMID: 16668973
Cooperative action of beta-glucan synthetase and UDP-xylose xylosyl transferase of Golgi membranes in the synthesis of xyloglucan-like polysaccharide.
Biochim Biophys Acta. 1980 May 22;629(3):431-44 PMID: 6448078
Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase.
Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12637-42 PMID: 8901635
beta-Glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn.
FEBS Lett. 1995 Nov 27;376(1-2):61-4 PMID: 8521968
Spatial organization of the assembly pathways of glycoproteins and complex polysaccharides in the Golgi apparatus of plants.
J Cell Biol. 1991 Feb;112(4):589-602 PMID: 1993733
Identification of the major starch synthase in the soluble fraction of potato tubers.
Plant Cell. 1996 Jul;8(7):1121-35 PMID: 8768372
Characterization of cDNAs encoding two isoforms of granule-bound starch synthase which show differential expression in developing storage organs of pea and potato.
Plant J. 1992 Mar;2(2):193-202 PMID: 1302049
Affinity chromatography of collagen glucosyltransferase on a UDP-glucose derivative coupled to agarose.
Biochim Biophys Acta. 1976 May 13;429(3):750-8 PMID: 817743
Effect of monensin on plant Golgi: re-examination of the monensin-induced changes in cisternal architecture and functional activities of the Golgi apparatus of sycamore suspension-cultured cells.
J Cell Sci. 1993 Mar;104 ( Pt 3):819-31 PMID: 8314876
Plant polypeptides reversibly glycosylated by UDP-glucose. Possible components of Golgi beta-glucan synthase in pea cells.
J Biol Chem. 1991 Nov 15;266(32):21977-84 PMID: 1834664

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.94.14.7679

Published

1997-07-08

Pages

7679-84

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC23882

Subset

Databases

GENBANK

U31565

External Links

PubMed source DOI: 10.1073/pnas.94.14.7679 PMC Full Text View journal details

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A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: purification, gene cloning, and trans-Golgi localization.

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