Solution structure of human CTLA-4 and delineation of a CD80/CD86 binding site conserved in CD28.

Nature structural biology ·Vol. 4 ·No. 7 ·1997-07-00 ·Pages 527-31

Metzler WJ, Bajorath J, Fenderson W, Shaw SY, Constantine KL, Naemura J, Leytze G, Peach RJ, Lavoie TB, Mueller L, Linsley PS

Abstract

The structure of human CTLA-4 reveals that residues Met 99, Tyr 100 and Tyr 104 of the M99YPPPY104 motif are adjacent to a patch of charged surface residues on the A'GFCC' face of the protein. Mutation of these residues, which are conserved in the CTLA-4/CD28 family, significantly reduces binding to CD80 and/or CD86, implicating this patch as a ligand binding site.

MeSH Terms

Abatacept Amino Acid Sequence Animals Antigens, CD/metabolism Antigens, Differentiation/chemistry,genetics,metabolism B7-1 Antigen/metabolism B7-2 Antigen Binding Sites CD28 Antigens/metabolism CTLA-4 Antigen Conserved Sequence Dimerization Humans Immunoconjugates Magnetic Resonance Spectroscopy/methods Membrane Glycoproteins/metabolism Models, Molecular Molecular Sequence Data Protein Conformation Sequence Homology, Amino Acid Solutions Sulfides

Chemicals

Antigens, CD Antigens, Differentiation B7-1 Antigen B7-2 Antigen CD28 Antigens CD86 protein, human CTLA-4 Antigen CTLA4 protein, human Immunoconjugates Membrane Glycoproteins Solutions Sulfides Abatacept

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Metzler W J

Bajorath J

Fenderson W

Shaw S Y

Constantine K L

Naemura J

Leytze G

Peach R J

Lavoie T B

Mueller L

Linsley P S

Article Info

Journal

Nature structural biology

Abbr.

Nat Struct Biol

ISSN

1072-8368

DOI

10.1038/nsb0797-527

Published

1997-07-00

Pages

527-31

Language

English

Region

United States

NLM ID

9421566

Subset

Databases

GENBANK

D49841, D49844, J02988, L15006, L22178, M34563, U37121, X05719, X55288, X67915, X93304, X93305

External Links

PubMed source DOI: 10.1038/nsb0797-527

Analysis Services

Solution structure of human CTLA-4 and delineation of a CD80/CD86 binding site conserved in CD28.

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