Abstract
Minimal potassium channel protein (minK) is a potassium channel protein consisting of 130 amino acids, possessing just one putative transmembrane domain. In this study we have synthesized a peptide with the amino acid sequence RDDSKLEALYILMVLGFFGFFTLGIMLSYI, containing the putative transmembrane region of minK, and analysed its secondary structure by using Fourier-transform IR and CD spectroscopy. The peptide was virtually insoluble in aqueous buffer, forming intermolecular beta-sheet aggregates. On attempted incorporation of the peptide into phospholipid membranes with a method involving dialysis, the peptide adopted a predominantly intermolecular beta-sheet conformation identical with that of the peptide in aqueous buffer, in agreement with a previous report [Horvàth, Heimburg, Kovachev, Findlay, Hideg and Marsh, (1995) Biochemistry 34, 3893-3898]. However, by using an alternative method of incorporating the peptide into phospholipid membranes we found that the peptide adopted a predominantly alpha-helical conformation, a finding consistent with various proposed structural models. These observed differences in secondary structure are due to artifacts of aggregation of the peptide before incorporation into lipid.
MeSH Terms
Amino Acid Sequence
Circular Dichroism
Lysophosphatidylcholines/chemistry
Membrane Lipids/chemistry
Membranes, Artificial
Molecular Sequence Data
Peptide Fragments/chemical synthesis,chemistry
Phospholipids/chemistry
Potassium Channels/chemistry
Protein Structure, Secondary
Spectroscopy, Fourier Transform Infrared
Chemicals
Lysophosphatidylcholines
Membrane Lipids
Membranes, Artificial
Peptide Fragments
Phospholipids
Potassium Channels
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mercer E A
Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K.
Abbott G W
Brazier S P
Ramesh B
Haris P I
Srai S K
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