Abstract
Protocols are presented for the preparation of a fully defined yeast RNA polymerase II transcription system, consisting of essentially pure TFIIB, -E, -F, and -H, TATA-binding protein, RNA polymerase II, and mediator of transcriptional regulation. This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins.
MeSH Terms
Carrier Proteins/chemistry,genetics
DNA-Binding Proteins/chemistry,genetics
Phosphoproteins/chemistry,genetics
RNA Polymerase II/chemistry,genetics
Saccharomyces cerevisiae/enzymology,genetics
Saccharomyces cerevisiae Proteins
TATA-Binding Protein Associated Factors
TATA-Box Binding Protein
Transcription Factor TFIIB
Transcription Factor TFIID
Transcription Factor TFIIH
Transcription Factors/chemistry,genetics
Transcription Factors, TFII
Transcription, Genetic
Chemicals
Carrier Proteins
DNA-Binding Proteins
Phosphoproteins
RNA polymerase II-binding proteins
Saccharomyces cerevisiae Proteins
TAF6 protein, S cerevisiae
TATA-Binding Protein Associated Factors
TATA-Box Binding Protein
Transcription Factor TFIIB
Transcription Factor TFIID
Transcription Factors
Transcription Factors, TFII
transcription factor TFIIE
Transcription Factor TFIIH
RNA Polymerase II
transcription factor TFIIF
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Myers L C
Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
Leuther K
Bushnell D A
Gustafsson C M
Kornberg R D