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PMID: 9237165 Published · ppublish English Journal Article

Yeast RNA polymerase II transcription reconstituted with purified proteins.

Methods (San Diego, Calif.) ·Vol. 12 ·No. 3 ·1997-07-00 ·Pages 212-6

Myers LC, Leuther K, Bushnell DA, Gustafsson CM, Kornberg RD

Abstract

Protocols are presented for the preparation of a fully defined yeast RNA polymerase II transcription system, consisting of essentially pure TFIIB, -E, -F, and -H, TATA-binding protein, RNA polymerase II, and mediator of transcriptional regulation. This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins.

MeSH Terms
Carrier Proteins/chemistry,genetics DNA-Binding Proteins/chemistry,genetics Phosphoproteins/chemistry,genetics RNA Polymerase II/chemistry,genetics Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIIB Transcription Factor TFIID Transcription Factor TFIIH Transcription Factors/chemistry,genetics Transcription Factors, TFII Transcription, Genetic
Chemicals
Carrier Proteins DNA-Binding Proteins Phosphoproteins RNA polymerase II-binding proteins Saccharomyces cerevisiae Proteins TAF6 protein, S cerevisiae TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIIB Transcription Factor TFIID Transcription Factors Transcription Factors, TFII transcription factor TFIIE Transcription Factor TFIIH RNA Polymerase II transcription factor TFIIF
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Myers L C
Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
Leuther K
Bushnell D A
Gustafsson C M
Kornberg R D
Article Info
Journal
Methods (San Diego, Calif.)
Abbr.
Methods
ISSN
1046-2023
Published
1997-07-00
Pages
212-6
Language
English
Region
United States
NLM ID
9426302
Subset
IM
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