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PMID: 9241256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions.

Nucleic acids research ·Vol. 25 ·No. 16 ·1997-08-15 ·Pages 3375-6

Kube DM, Srivastava A

Abstract

Titers of wild-type and recombinant adeno-associated viruses are routinely determined by DNA slot blot analysis. The binding of viral DNA to nylon membranes was found to be inhibited by magnesium ions, which are critical components of the DNase I digestion carried out prior to slot blot analysis. Mg2+ions also interfered with the adsorption of plasmid DNA to nylon and nitrocellulose membranes. These observations yield practical insights into the poorly understood mechanisms by which DNA molecules are retained on solid supports.

MeSH Terms
Adsorption Calcium/metabolism Collodion DNA, Viral/analysis Dependovirus/genetics Magnesium/metabolism Membranes, Artificial Nucleic Acid Hybridization/methods Nylons
Chemicals
DNA, Viral Membranes, Artificial Nylons Collodion Magnesium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kube D M
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Srivastava A
References (9)
9 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.16.3375
Published
1997-08-15
Pages
3375-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146885
Subset
IM
Grants
NIDDK NIH HHS · DK-49218 · United States
NHLBI NIH HHS · HL-48342 · United States
NHLBI NIH HHS · HL-53586 · United States
External Links
PubMed source DOI: 10.1093/nar/25.16.3375 PMC Full Text View journal details
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