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PMID: 92573 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Establishment of a lymphoblastoid cell line and isolation of an Epstein-Barr-related virus of gorilla origin.

Journal of virology ·Vol. 31 ·No. 3 ·1979-09-00 ·Pages 845-8

Neubauer RH, Rabin H, Strnad BC, Nonoyama M, Nelson-Rees WA

Abstract

A B-lymphoid cell line was established from a normal gorilla. The cells contained Epstein-Barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. DNA-DNA reassociation kinétics revealed 30 to 40% hybridization to Epstein-Barr virus with 50 genomes per cell. Examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed Herpesvirus gorilla. H. gorilla transformed gibbon B-lymphocytes in vitro.

MeSH Terms
Animals Antigens, Viral/analysis B-Lymphocytes/microbiology Blood/microbiology Cell Line DNA, Viral/analysis Epitopes Female Gorilla gorilla/microbiology Herpesviridae/analysis,immunology,isolation & purification Herpesvirus 4, Human/analysis,immunology Humans Lymphocyte Activation Nucleic Acid Conformation Nucleic Acid Hybridization
Chemicals
Antigens, Viral DNA, Viral Epitopes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Neubauer R H
Rabin H
Strnad B C
Nonoyama M
Nelson-Rees W A
References (16)
16 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.31.3.845-848.1979
Published
1979-09-00
Pages
845-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353513
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.31.3.845-848.1979 PMC Full Text View journal details
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