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PMID: 9261401 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effects of nucleocapsid mutations on human immunodeficiency virus assembly and RNA encapsidation.

Journal of virology ·Vol. 71 ·No. 9 ·1997-09-00 ·Pages 6765-76

Zhang Y, Barklis E

Abstract

The human immunodeficiency virus (HIV) Pr55Gag precursor proteins direct virus particle assembly. While Gag-Gag protein interactions which affect HIV assembly occur in the capsid (CA) domain of Pr55Gag, the nucleocapsid (NC) domain, which functions in viral RNA encapsidation, also appears to participate in virus assembly. In order to dissect the roles of the NC domain and the p6 domain, the C-terminal Gag protein domain, we examined the effects of NC and p6 mutations on virus assembly and RNA encapsidation. In our experimental system, the p6 domain did not appear to affect virus release efficiency but p6 deletions and truncations reduced the specificity of genomic HIV-1 RNA encapsidation. Mutations in the nucleocapsid region reduced particle release, especially when the p2 interdomain peptide or the amino-terminal portion of the NC region was mutated, and NC mutations also reduced both the specificity and the efficiency of HIV-1 RNA encapsidation. These results implicated a linkage between RNA encapsidation and virus particle assembly or release. However, we found that the mutant ApoMTRB, in which the nucleocapsid and p6 domains of HIV-1 Pr55Gag were replaced with the Bacillus subtilis MtrB protein domain, released particles efficiently but packaged no detectable RNA. These results suggest that, for the purposes of virus-like particle assembly and release, NC can be replaced by a protein that does not appear to encapsidate RNA.

MeSH Terms
Animals COS Cells Gene Products, gag/genetics,metabolism HIV-1/genetics,physiology Humans Mutation Nucleocapsid/genetics,metabolism Protein Precursors/genetics,metabolism RNA, Viral/metabolism Virion Virus Assembly gag Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, gag Protein Precursors RNA, Viral gag Gene Products, Human Immunodeficiency Virus gag protein p1, Human immunodeficiency virus p55 gag precursor protein, Human immunodeficiency virus 1 p6 gag protein, Human immunodeficiency virus 1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhang Y
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201-3098, USA.
Barklis E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.9.6765-6776.1997
Published
1997-09-00
Pages
6765-76
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191957
Subset
IM
Grants
NCI NIH HHS · 2RO1CA47088-07 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.9.6765-6776.1997 PMC Full Text View journal details
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