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PMID: 9265642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant.

The Journal of cell biology ·Vol. 138 ·No. 4 ·1997-08-25 ·Pages 731-46

Luo Wj, Chang A

Abstract

A novel genetic selection was used to identify genes regulating traffic in the yeast endosomal system. We took advantage of a temperature-sensitive mutant in PMA1, encoding the plasma membrane ATPase, in which newly synthesized Pma1 is mislocalized to the vacuole via the endosome. Diversion of mutant Pma1 from vacuolar delivery and rerouting to the plasma membrane is a major mechanism of suppression of pma1(ts). 16 independent suppressor of pma1 (sop) mutants were isolated. Identification of the corresponding genes reveals eight that are identical with VPS genes required for delivery of newly synthesized vacuolar proteins. A second group of SOP genes participates in vacuolar delivery of mutant Pma1 but is not essential for delivery of the vacuolar protease carboxypeptidase Y. Because the biosynthetic pathway to the vacuole intersects with the endocytic pathway, internalization of a bulk membrane endocytic marker FM 4-64 was assayed in the sop mutants. By this means, defective endosome-to-vacuole trafficking was revealed in a subset of sop mutants. Another subset of sop mutants displays perturbed trafficking between endosome and Golgi: impaired pro-alpha factor processing in these strains was found to be due to defective recycling of the trans-Golgi protease Kex2. One of these strains defective in Kex2 trafficking carries a mutation in SOP2, encoding a homologue of mammalian synaptojanin (implicated in synaptic vesicle endocytosis and recycling). Thus, cell surface delivery of mutant Pma1 can occur as a consequence of disturbances at several different sites in the endosomal system.

MeSH Terms
Biological Transport/genetics Biomarkers Carrier Proteins/genetics Cell Membrane/enzymology,genetics,metabolism Endosomes/enzymology,genetics,metabolism Fungal Proteins/genetics Intracellular Membranes/enzymology Mating Factor Mutagenesis Nerve Tissue Proteins/genetics Peptides/genetics Phosphoric Monoester Hydrolases/genetics Proprotein Convertases Protein Processing, Post-Translational Proton-Translocating ATPases/genetics Receptors, Cell Surface/genetics Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Subtilisins/metabolism Vacuoles/chemistry,enzymology,genetics Vesicular Transport Proteins
Chemicals
Biomarkers Carrier Proteins Fungal Proteins Nerve Tissue Proteins PEP1 protein, S cerevisiae PMA2 protein, S cerevisiae Peptides Receptors, Cell Surface Saccharomyces cerevisiae Proteins Vesicular Transport Proteins Mating Factor synaptojanin Phosphoric Monoester Hydrolases Proprotein Convertases Subtilisins KEX2 protein, S cerevisiae PMA1 protein, S cerevisiae Proton-Translocating ATPases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Luo W j
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Chang A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.138.4.731
Published
1997-08-25
Pages
731-46
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2138039
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.138.4.731 PMC Full Text View journal details
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