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PMID: 9266176 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.

Current opinion in structural biology ·Vol. 7 ·No. 4 ·1997-08-00 ·Pages 537-42

Kaback HR, Voss J, Wu J

Abstract

Recent advances in protein engineering have facilitated the development of alternative approaches to determine helix packing in polytopic membrane proteins. Using the lac permease as a paradigm, several site-directed biophysical and biochemical techniques are described which should be generally applicable.

MeSH Terms

Amino Acid Sequence Escherichia coli/enzymology Escherichia coli Proteins Membrane Proteins/chemistry Membrane Transport Proteins/chemistry Models, Molecular Molecular Sequence Data Monosaccharide Transport Proteins Mutagenesis, Site-Directed Protein Structure, Secondary Protein Structure, Tertiary Symporters

Chemicals

Escherichia coli Proteins LacY protein, E coli Membrane Proteins Membrane Transport Proteins Monosaccharide Transport Proteins Symporters lactose permease

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kaback H R

Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024-1662, USA. [email protected]

Voss J

Wu J

Article Info

Journal

Current opinion in structural biology

Abbr.

Curr Opin Struct Biol

ISSN

0959-440X

DOI

10.1016/s0959-440x(97)80119-4

[email protected]

Published

1997-08-00

Pages

537-42

Language

English

Region

England

NLM ID

9107784

Subset

Grants

NIDDK NIH HHS · 1 R01 DK51131-01 · United States

External Links

PubMed source DOI: 10.1016/s0959-440x(97)80119-4 View journal details

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Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.

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