Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

Nucleic acids research ·Vol. 25 ·No. 18 ·1997-09-15 ·Pages 3747-8

Kinoshita Y, Nishigaki K, Husimi Y

Abstract

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.

MeSH Terms

Biotin DNA/chemistry Fluorescent Dyes Isotope Labeling Molecular Probe Techniques RNA/chemistry RNA Ligase (ATP)

Chemicals

Fluorescent Dyes RNA Biotin DNA RNA Ligase (ATP)

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kinoshita Y

Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa 338, Japan.

Nishigaki K

Husimi Y

References (12)

12 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/25.18.3747

Published

1997-09-15

Pages

3747-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC146945

Subset

External Links

PubMed source DOI: 10.1093/nar/25.18.3747 PMC Full Text View journal details

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Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

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