Home LiteratureArticle Details

PMID: 9278503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The complete genome sequence of Escherichia coli K-12.

Science (New York, N.Y.) ·Vol. 277 ·No. 5331 ·1997-09-05 ·Pages 1453-62

Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y

Abstract

The 4,639,221-base pair sequence of Escherichia coli K-12 is presented. Of 4288 protein-coding genes annotated, 38 percent have no attributed function. Comparison with five other sequenced microbes reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident. The largest family of paralogous proteins contains 80 ABC transporters. The genome as a whole is strikingly organized with respect to the local direction of replication; guanines, oligonucleotides possibly related to replication and recombination, and most genes are so oriented. The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer.

MeSH Terms

Bacterial Proteins/chemistry,genetics,metabolism Bacteriophage lambda/genetics Base Composition Binding Sites Chromosome Mapping DNA Replication DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genome, Bacterial Molecular Sequence Data Mutation Operon RNA, Bacterial/genetics RNA, Transfer/genetics Recombination, Genetic Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Sequence Homology, Amino Acid

Chemicals

Bacterial Proteins DNA Transposable Elements DNA, Bacterial RNA, Bacterial RNA, Transfer

Authors & Affiliations

17 authors, click to expand affiliations / ORCID

Blattner F R

Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, WI 53706, USA. [email protected]

Plunkett G

Bloch C A

Perna N T

Burland V

Riley M

Collado-Vides J

Glasner J D

Rode C K

Mayhew G F

Gregor J

Davis N W

Kirkpatrick H A

Goeden M A

Rose D J

Mau B

Shao Y

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.277.5331.1453

[email protected]

Published

1997-09-05

Pages

1453-62

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NHGRI NIH HHS · P01 HG01428 · United States

NCRR NIH HHS · S10 RR10379 · United States

Databases

GENBANK

AE000111, AE000112, AE000113, AE000114, AE000115, AE000116, AE000117, AE000118, AE000119, AE000120, AE000121, AE000122, AE000123, AE000124, AE000125, AE000126, AE000127, AE000128, AE000129, AE000130, AE000131, AE000132, AE000133, AE000134, AE000135, AE000136, AE000137

Corrections

CommentIn

PMID: 9304210

External Links

PubMed source DOI: 10.1126/science.277.5331.1453 View journal details

Analysis Services

The complete genome sequence of Escherichia coli K-12.

Contact

WeChat Official Account