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PMID: 9290646 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and expression of a PR5-like protein from Arabidopsis: inhibition of fungal growth by bacterially expressed protein.

Plant molecular biology ·Vol. 34 ·No. 6 ·1997-08-00 ·Pages 949-59

Hu X, Reddy AS

Abstract

Pathogenesis-related (PR)-5 proteins are a family of proteins that are induced by different phytopathogens in many plants and share significant sequence similarity with thaumatin. We isolated a complementary DNA (ATLP-3) encoding a PR5-like protein from Arabidopsis which is distinct from two other previously reported PR5 cDNAs from the same plant species. The predicted ATLP-3 protein with its amino-terminal signal sequence is 245 amino acids in length and is acidic with a pl of 4.8. The deduced amino acid sequence of ATLP-3 shows significant sequence similarity with PR5 and thaumatin-like proteins from Arabidopsis and other plants and contains a putative signal sequence at the amino-terminus. The expression of ATLP-3 and a related gene (ATLP-1) that we previously isolated from Arabidopsis was induced by pathogen infection and salicylic acid, a known inducer of pathogenesis-related genes. Southern blot analysis indicates that the ATLP-1 and ATLP-3 are coded by single-copy genes. To study the effect of ATLP-1 and ATLP-3 proteins on fungal growth, the cDNA regions corresponding to putative mature protein were expressed in Escherichia coli and the cDNA encoded proteins were purified. ATLP-1 and ATLP-3 proteins cross-reacted with anti-osmotin and anti-zeamatin antibodies. ATLP-3 protein showed antifungal activity against several fungal pathogens suggesting that ATLP-3 may be involved in plant defense against fungal pathogens.

MeSH Terms
Amino Acid Sequence Antifungal Agents/pharmacology Arabidopsis/genetics Base Sequence Blotting, Northern Blotting, Southern Candida albicans/drug effects Cloning, Molecular DNA, Complementary/genetics Escherichia coli/genetics Microbial Sensitivity Tests Mitosporic Fungi/drug effects Molecular Sequence Data Plant Diseases/genetics Plant Proteins/biosynthesis,genetics,pharmacology Recombinant Proteins/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Sweetening Agents
Chemicals
Antifungal Agents DNA, Complementary Plant Proteins Recombinant Proteins Sweetening Agents pathogenesis-related proteins, plant thaumatin protein, plant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hu X
Department of Biology, Colorado State University, Fort Collins 80523, USA.
Reddy A S
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005893119263
Published
1997-08-00
Pages
949-59
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U83490
External Links
PubMed source DOI: 10.1023/a:1005893119263 View journal details
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