Immunocytochemical technique for detection of prolactin (PRL) and growth hormone (GH) in hyperplastic and neoplastic lesions of dog prostate and mammary gland.

Histochemistry ·Vol. 64 ·No. 3 ·1979-00-00 ·Pages 279-86

El Etreby MF, Mahrous AT

Abstract

An immunocytochemical technique was described to test for immunoreactive prolactin (PRL) and growth hormone (GH) in spontaneous and experimentally induced hyperplastic and neoplastic lesions of the prostate and mammary gland. The dog was used as an animal model. The specificity and validity of the immunocytochemical staining procedure and of the antisera to canine PRL and canine GH can be regarded as established for the demonstration of PRL- and GH-dependent staining respectively. In mammary and prostatic tissues, both endogenous PRL and GH as well as intracellular free binding sites (for exogenous PRL and GH) were detected immunocytochemically. The technique presented seems to be an important tool to localize putative target sites for pituitary hormones in hormone-dependent hyperplasia and neoplasia.

MeSH Terms

Androstenediol/pharmacology Animals Castration Cyproterone/pharmacology Dogs Estradiol/pharmacology Female Growth Hormone/analysis Immunoenzyme Techniques Male Mammary Glands, Animal/pathology Mammary Neoplasms, Experimental/analysis,pathology Prolactin/analysis Prostate/drug effects,pathology Prostatic Hyperplasia/pathology Prostatic Neoplasms/analysis,pathology

Chemicals

Estradiol Prolactin Growth Hormone Androstenediol Cyproterone

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

El Etreby M F

Mahrous A T

References (25)

25 references, click to expand

Evaluation of effects of sexual steroids on the hypothalamic-pituitary system of animals and man.
Arch Toxicol Suppl. 1979;(2):11-39 PMID: 380519
Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.
J Cell Biol. 1967 May;33(2):307-18 PMID: 6046571
Demonstration of gonadotropin binding sites in the rat ovary by an immunoglobulin-enzyme bridge method.
Eur J Obstet Gynecol Reprod Biol. 1974;4(1 SUPPL):S3-9 PMID: 4606413
Role of the pituitary gland in experimental hormonal induction and prevention of benign prostatic hyperplasia in the dog.
Cell Tissue Res. 1979;204(3):367-78 PMID: 93515
Effect of contraceptive steroids on mammary gland of beagle dog and its relevance to human carcinogenicity.
Pharmacol Ther B. 1979;5(1-3):369-402 PMID: 386375
Detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation.
Endocrinology. 1976 Oct;99(4):949-58 PMID: 789055
Localization of thyrotropin (TSH) in the dog pituitary gland.
Cell Tissue Res. 1978 Jan 31;186(3):399-412 PMID: 75068
Intracellular localization of parathyroid hormone in the kidney.
Endocrinology. 1974 Jul;95(1):229-37 PMID: 4835881
Quantitative immunocytochemistry of pituitary receptors for luteinizing hormone-releasing hormone.
Cell Tissue Res. 1975 Sep 17;162(2):141-76 PMID: 52407
Intracellular prolactin in rat corpus luteum and adrenal cortex.
Endocrinology. 1978 Feb;102(2):402-6 PMID: 743964
Role of lactogen in prostatic physiology.
Urol Res. 1975 Oct 29;3(3):129-32 PMID: 1189140
Sex hormone binding globulin binding capacity, testosterone, 5alpha-dihydrotestosterone, oestradiol and prolactin in plasma of patients with prostatic carcinoma under various types of hormonal treatment.
Acta Endocrinol (Copenh). 1977 Jul;85(3):650-64 PMID: 325982
Localization of gonadotrophic hormones in the dog pituitary gland. A study using immunoenzyme histochemistry and chemical staining.
Cell Tissue Res. 1977 Sep 26;183(2):167-75 PMID: 72609
Endocrinology of reproduction in the female beagle dog and its significance in mammary gland tumorigenesis.
Acta Endocrinol Suppl (Copenh). 1979;222:1-34 PMID: 285559
Electron microscopic study of the localization of labeled gonadotropins in the Sertoli and Leydig cells of the rat testis.
Proc Soc Exp Biol Med. 1970 Feb;133(2):582-6 PMID: 5414244
Adenohypophysis: ultrastructural cytochemistry. A review.
J Histochem Cytochem. 1973 Oct;21(10):855-94 PMID: 4356692
BASIC EXPERIMENTAL STUDIES OF THE PITUITARY PROLACTIN-PROSTATE INTER-RELATIONSHIPS.
J Urol. 1965 Jan;93:87-93 PMID: 14254366
The role of the pituitary gland in spontaneous canine mammary tumorigenesis.
Vet Pathol. 1980 Jan;17(1):2-16 PMID: 7352360
The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.
J Histochem Cytochem. 1970 May;18(5):315-33 PMID: 4192899
Evidence for androgen-dependent intracellular binding of prolactin in rat ventral prostate gland.
Endocrinology. 1977 Sep;101(3):929-38 PMID: 70348
Immunohistochemical studies of prolactin binding in sex accessory organs of the male rat.
J Histochem Cytochem. 1978 Jul;26(7):565-80 PMID: 357642
Effect of cyproterone acetate, d-norgestrel and progesterone on the canine mammary gland.
Cell Tissue Res. 1978 Nov 20;194(2):245-67 PMID: 728963
Localization of prolactin in rat kidney tissue using a double-antibody technique.
J Endocrinol. 1975 Jul;66(1):101-6 PMID: 1100747
An immunoglobulin-enzyme bridge method for localizing tissue antigens.
J Histochem Cytochem. 1969 Sep;17(9):563-9 PMID: 4186250
The utility of antisera to canine growth hormone and canine prolactin for immunocytochemical staining of the dog pituitary gland.
Histochemistry. 1977 Jul 18;53(1):1-15 PMID: 71286

Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00495028

Published

1979-00-00

Pages

279-86

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00495028

Analysis Services

Immunocytochemical technique for detection of prolactin (PRL) and growth hormone (GH) in hyperplastic and neoplastic lesions of dog prostate and mammary gland.

Contact

WeChat Official Account