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PMID: 9311921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

NF-AT activation induced by a CAML-interacting member of the tumor necrosis factor receptor superfamily.

Science (New York, N.Y.) ·Vol. 278 ·No. 5335 ·1997-10-03 ·Pages 138-41

von Bülow GU, Bram RJ

Abstract

Activation of the nuclear factor of activated T cells transcription factor (NF-AT) is a key event underlying lymphocyte action. The CAML (calcium-modulator and cyclophilin ligand) protein is a coinducer of NF-AT activation when overexpressed in Jurkat T cells. A member of the tumor necrosis factor receptor superfamily was isolated by virtue of its affinity for CAML. Cross-linking of this lymphocyte-specific protein, designated TACI (transmembrane activator and CAML-interactor), on the surface of transfected Jurkat cells with TACI-specific antibodies led to activation of the transcription factors NF-AT, AP-1, and NFkappaB. TACI-induced activation of NF-AT was specifically blocked by a dominant-negative CAML mutant, thus implicating CAML as a signaling intermediate.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Calcineurin Calmodulin-Binding Proteins/metabolism Carrier Proteins/genetics,metabolism Cell Line Cell Membrane/metabolism DNA-Binding Proteins/metabolism Humans Jurkat Cells Lymphocyte Activation Membrane Proteins Molecular Sequence Data Mutation NF-kappa B/metabolism NFATC Transcription Factors Nuclear Proteins Phosphoprotein Phosphatases/metabolism Receptors, Tumor Necrosis Factor/chemistry,genetics,metabolism Sequence Alignment Signal Transduction T-Lymphocytes/immunology,metabolism Transcription Factor AP-1/metabolism Transcription Factors/metabolism Transcription, Genetic Transfection Transmembrane Activator and CAML Interactor Protein
Chemicals
Adaptor Proteins, Signal Transducing CAMLG protein, human Calmodulin-Binding Proteins Carrier Proteins DNA-Binding Proteins Membrane Proteins NF-kappa B NFATC Transcription Factors Nuclear Proteins Receptors, Tumor Necrosis Factor TNFRSF13B protein, human Transcription Factor AP-1 Transcription Factors Transmembrane Activator and CAML Interactor Protein Calcineurin Phosphoprotein Phosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
von Bülow G U
Department of Experimental Oncology, St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA.
Bram R J
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
Published
1997-10-03
Pages
138-41
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NCI NIH HHS · CA21765 · United States
Databases
GENBANK
AF023614
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