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PMID: 9316944 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans.

Journal of clinical microbiology ·Vol. 35 ·No. 10 ·1997-10-00 ·Pages 2709-11

Roberts B, Hirst R

Abstract

We have developed a technique based on the use of monodisperse magnetic beads to isolate Mycobacterium ulcerans from heterogenous mixtures, prior to PCR amplification. Using this method, we were able to detect M. ulcerans in water samples taken from Phillip Island, Australia, the site of several outbreaks of M. ulcerans disease in recent times.

MeSH Terms
Antibodies, Bacterial Australia/epidemiology Disease Outbreaks Fresh Water Immunomagnetic Separation/methods Mycobacterium Infections, Nontuberculous/epidemiology Mycobacterium ulcerans/immunology,isolation & purification Polymerase Chain Reaction/methods Water Microbiology
Chemicals
Antibodies, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roberts B
Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia. [email protected]
Hirst R
References (7)
7 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.35.10.2709-2711.1997
Email
[email protected]
Published
1997-10-00
Pages
2709-11
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC230047
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.35.10.2709-2711.1997 PMC Full Text View journal details
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