A simple method of lymphocyte purification from human peripheral blood.

We present a simple technique for obtaining cell suspension from human peripheral venous blood containing on average 96% lymphocytes, no macrophages and leukocyte red blood cell ratio 1 : 1. Cells are readily prepared under sterile conditions and can be used in culture experiments. The high yield of lymphocytes and the avoidance of Ficoll-Triosil makes the technique especially valuable for micro cytotoxicity tests such as those aimed at measuring tumour immunity.