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PMID: 9326584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 21 ·1997-10-14 ·Pages 11190-5

Lingner J, Cech TR, Hughes TR, Lundblad V

Abstract

Telomerase is a specialized reverse transcriptase consisting of both RNA and protein components. Previous characterization of yeast telomerase function in vivo identified four EST (for ever shorter telomeres) genes that, when mutated, result in the phenotypes expected for a defect in telomerase. Consistent with this genetic prediction, the EST2 gene has recently been shown to encode the catalytic component of telomerase. Using an in vitro assay, we show here that telomerase activity is present in extracts prepared from yeast strains carrying est1-Delta, est3-Delta, and cdc13-2(est) mutations. Therefore, while these three genes are necessary for telomerase function in vivo, they do not encode components essential for core catalytic activity. When Est2p, the one EST gene product found to be essential for catalytic activity, was immunoprecipitated from extracts, the telomerase RNA subunit was also specifically precipitated, supporting the conclusion that these two components are in a stable complex.

MeSH Terms
Centrifugation, Density Gradient Cyclin B/genetics,metabolism Fungal Proteins/genetics,metabolism Genes, Fungal Mutagenesis Polymerase Chain Reaction Proteins/genetics,metabolism Saccharomyces cerevisiae/enzymology,genetics,growth & development Saccharomyces cerevisiae Proteins Telomerase/genetics,isolation & purification,metabolism Telomere/genetics Telomere-Binding Proteins
Chemicals
Cdc13 protein, S cerevisiae Cyclin B Fungal Proteins Proteins Saccharomyces cerevisiae Proteins Telomere-Binding Proteins EST1 protein, S cerevisiae EST3 protein, S cerevisiae Telomerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lingner J
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.
Cech T R
Hughes T R
Lundblad V
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.21.11190
Published
1997-10-14
Pages
11190-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23412
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.94.21.11190 PMC Full Text View journal details
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