Estimation of membrane potentials of individual lymphocytes by flow cytometry.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 11 ·1979-11-00 ·Pages 5728-30

Shapiro HM, Natale PJ, Kamentsky LA

Abstract

The membrane potentials of individual cells can be estimated by flow cytometric quantitation of the cells' uptake of the fluorescent lipophilic cationic dye 3,3'-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients are uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarize only a fraction of the lymphocytes. The flow cytometric technique allows precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.

MeSH Terms

Cations Cell Separation/methods Coloring Agents Gramicidin/pharmacology Humans Lectins/pharmacology Lymphocytes/drug effects,physiology Membrane Potentials/drug effects Spectrometry, Fluorescence Valinomycin/pharmacology

Chemicals

Cations Coloring Agents Lectins Gramicidin Valinomycin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Shapiro H M

Natale P J

Kamentsky L A

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.11.5728

Published

1979-11-00

Pages

5728-30

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411723

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.11.5728 PMC Full Text View journal details

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Estimation of membrane potentials of individual lymphocytes by flow cytometry.

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