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PMID: 9336459 Published · ppublish English Journal Article

The expression of a novel, epithelium-specific ets transcription factor is restricted to the most differentiated layers in the epidermis.

Nucleic acids research ·Vol. 25 ·No. 21 ·1997-11-01 ·Pages 4287-95

Andreoli JM, Jang SI, Chung E, Coticchia CM, Steinert PM, Markova NG

Abstract

Ets proteins have been implicated in the regulation of gene expression during a variety of biological processes, including growth control, differentiation, development and transformation. More than 35 related proteins containing the 'ets domain' have now been found which specifically interact with DNA sequences encompassing the core tetranucleotide GGAA. Although ets responsive genes have been identified in the epidermis, little is known about their distribution and function in this tissue. We have now demonstrated that epidermis and cultured epidermal keratinocytes synthesize numerous ets proteins. The expression of some of these proteins is regulated as a function of differentiation. Among these is a novel ets transcription factor with a dual DNA-binding specificity, which we have called jen. The expression of jen is not only epithelial specific, but it is the only ets protein so far described, and one of the very few transcription factors whose expression is restricted to the most differentiated epidermal layers. We show that two epidermal marker genes whose expression coincides with that of jen are transregulated by this protein in a complex mode which involves interactions with other transcriptional regulators such as Sp1 and AP1.

MeSH Terms
Adult Amino Acid Sequence Base Sequence Cell Differentiation Cells, Cultured Cloning, Molecular DNA/metabolism DNA, Complementary/genetics DNA-Binding Proteins Epidermal Cells Epidermis/metabolism Epithelial Cells/metabolism Filaggrin Proteins Gene Expression Regulation, Developmental Humans Intermediate Filament Proteins/genetics Keratinocytes/chemistry Molecular Sequence Data Protein Precursors/genetics Proto-Oncogene Proteins/biosynthesis,chemistry,genetics Proto-Oncogene Proteins c-ets RNA, Messenger/analysis Sequence Analysis, DNA Sequence Homology, Amino Acid Skin/metabolism Trans-Activators/biosynthesis,chemistry,genetics Transcription Factors/biosynthesis,chemistry,genetics
Chemicals
DNA, Complementary DNA-Binding Proteins ELF3 protein, human Filaggrin Proteins Intermediate Filament Proteins Protein Precursors Proto-Oncogene Proteins Proto-Oncogene Proteins c-ets RNA, Messenger Trans-Activators Transcription Factors DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Andreoli J M
Laboratory of Skin Biology, NIAMS, NIH, Bethesda, MD 20892-2752, USA.
Jang S I
Chung E
Coticchia C M
Steinert P M
Markova N G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.21.4287
Published
1997-11-01
Pages
4287-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147045
Subset
IM
Databases
GENBANK
U97156
External Links
PubMed source DOI: 10.1093/nar/25.21.4287 PMC Full Text View journal details
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