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PMID: 9368418 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of a Cs(+)-resistant guard cell K+ channel confers Cs(+)-resistant, light-induced stomatal opening in transgenic arabidopsis.

The Plant cell ·Vol. 9 ·No. 10 ·1997-10-00 ·Pages 1843-57

Ichida AM, Pei ZM, Baizabal-Aguirre VM, Turner KJ, Schroeder JI

Abstract

Inward-rectifying K+ (K+in) channels in the guard cell plasma membrane have been suggested to function as a major pathway for K+ influx into guard cells during stomatal opening. When K+in channels were blocked with external Cs+ in wild-type Arabidopsis guard cells, light-induced stomatal opening was reduced. Transgenic Arabidopsis plants were generated that expressed a mutant of the guard cell K+in channel, KAT1, which shows enhanced resistance to the Cs+ block. Stomata in these transgenic lines opened in the presence of external Cs+. Patch-clamp experiments with transgenic guard cells showed that inward K+(in) currents were blocked less by Cs+ than were K+ currents in controls. These data provide direct evidence that KAT1 functions as a plasma membrane K+ channel in vivo and that K+in channels constitute an important mechanism for light-induced stomatal opening. In addition, biophysical properties of K+in channels in guard cells indicate that components in addition to KAT1 may contribute to the formation of K+in channels in vivo.

MeSH Terms
Arabidopsis/physiology,radiation effects Cesium/pharmacology Gene Expression Ion Channel Gating Patch-Clamp Techniques Plants, Genetically Modified/physiology,radiation effects Potassium Channels/drug effects,genetics
Chemicals
Potassium Channels Cesium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ichida A M
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
Pei Z M
Baizabal-Aguirre V M
Turner K J
Schroeder J I
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.9.10.1843
Published
1997-10-00
Pages
1843-57
Language
English
Region
England
NLM ID
9208688
PMCID
PMC157026
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.9.10.1843 PMC Full Text View journal details
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