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PMID: 9380722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 20 ·1997-09-30 ·Pages 10844-9

Tsitsikov EN, Gutierrez-Ramos JC, Geha RS

Abstract

The tetraspanin CD81 is ubiquitously expressed and associated with CD19 on B lymphocytes and with CD4 and CD8 on T lymphocytes. Analysis of mice with disrupted CD81 gene reveals normal T cells but a distinct abnormality in B cells consisting of decreased expression of CD19 and severe reduction in peritoneal B-1 cells. CD81-deficient B cells responded normally to surface IgM crosslinking, but had severely impaired calcium influx following CD19 engagement. CD81-deficient mice had increased serum IgM and IgA and an exaggerated antibody response to the type II T independent antigen TNP-Ficoll. These results suggest that CD81 is important for CD19 signaling and B cell function.

MeSH Terms
Animals Antibody Formation Antigens, CD/genetics Antigens, CD19/genetics,metabolism Antigens, T-Independent/immunology B-Lymphocytes/cytology,immunology Immunoglobulin A/blood Immunoglobulin M/blood Lymphocyte Depletion Membrane Proteins Mice Signal Transduction Tetraspanin 28
Chemicals
Antigens, CD Antigens, CD19 Antigens, T-Independent Cd81 protein, mouse Immunoglobulin A Immunoglobulin M Membrane Proteins Tetraspanin 28
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsitsikov E N
Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
Gutierrez-Ramos J C
Geha R S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.20.10844
Published
1997-09-30
Pages
10844-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23503
Subset
IM
Grants
NICHD NIH HHS · HD 17461 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.20.10844 PMC Full Text View journal details
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