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PMID: 938677 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The attachment and penetration of T7 DNA/phage in Syrian hamster embryonic cells.

Biochimica et biophysica acta ·Vol. 435 ·No. 2 ·1976-06-18 ·Pages 167-83

Leavitt JC, Schechtman LM, Ts'o PO, Borenfreund E, Bendich A

Abstract

Bacteriophage T7 DNA can penetrate Syrian hamster embryonic cells after a mandatory initial pretreatment with DEAE-dextran. In 3 h an extracellular complex between T7DNA and the cell monolayer is formed which is equivalent to 105 T7 genomes per cell. During the ensuing 24-48 h of cell growth, an average of 102-103 T7 genomes are transported to the nucleus in 90% of the cells of the culture.

MeSH Terms

Binding Sites Biological Transport Cell Line Cell Nucleus/metabolism,ultrastructure Coliphages/metabolism DNA Replication DNA, Viral/metabolism Deoxyribonucleases Genes Kinetics Molecular Weight Nucleic Acid Denaturation Nucleic Acid Hybridization Virus Replication

Chemicals

DNA, Viral Deoxyribonucleases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Leavitt J C

Schechtman L M

Ts'o P O

Borenfreund E

Bendich A

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2787(76)90248-3

Published

1976-06-18

Pages

167-83

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0005-2787(76)90248-3

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The attachment and penetration of T7 DNA/phage in Syrian hamster embryonic cells.

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