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PMID: 9401038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A glgC gene essential only for the first of two spatially distinct phases of glycogen synthesis in Streptomyces coelicolor A3(2).

Journal of bacteriology ·Vol. 179 ·No. 24 ·1997-12-00 ·Pages 7784-9

Martin MC, Schneider D, Bruton CJ, Chater KF, Hardisson C

Abstract

By using a PCR approach based on conserved regions of ADP-glucose pyrophosphorylases, a glgC gene was cloned from Streptomyces coelicolor A3(2). The deduced glgC gene product showed end-to-end relatedness to other bacterial ADP-glucose pyrophosphorylases. The glgC gene is about 1,000 kb from the leftmost chromosome end and is not closely linked to either of the two glgB genes of S. coelicolor, which encode glycogen branching enzymes active in different locations in differentiated colonies. Disruption of glgC eliminated only the first of two temporal peaks of ADP-glucose pyrophosphorylase activity and glycogen accumulation and prevented cytologically observable glycogen accumulation in the substrate mycelium of colonies (phase I), while glycogen deposition in young spore chains (phase II) remained readily detectable. The cloned glgC gene therefore encodes an ADP-glucose pyrophosphorylase essential only for phase I (and it is therefore named glgCI). A second, phase II-specific, glgC gene should also exist in S. coelicolor, though it was not detected by hybridization analysis.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosome Mapping Cloning, Molecular Genes, Bacterial Glucose-1-Phosphate Adenylyltransferase Glycogen/biosynthesis,isolation & purification Molecular Sequence Data Mutagenesis Nucleotidyltransferases/genetics Streptomyces/enzymology,genetics,growth & development,ultrastructure
Chemicals
Bacterial Proteins Glycogen Nucleotidyltransferases Glucose-1-Phosphate Adenylyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Martin M C
Area de Microbiología, Facultad de Medicina and Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, Spain.
Schneider D
Bruton C J
Chater K F
Hardisson C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.24.7784-7789.1997
Published
1997-12-00
Pages
7784-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179742
Subset
IM
Databases
GENBANK
X89733
External Links
PubMed source DOI: 10.1128/jb.179.24.7784-7789.1997 PMC Full Text View journal details
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