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PMID: 9426629 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and characterisation of MEK1, an Arabidopsis gene encoding a homologue of MAP kinase kinase.

Plant molecular biology ·Vol. 35 ·No. 6 ·1997-12-00 ·Pages 1057-64

Morris PC, Guerrier D, Leung J, Giraudat J

Abstract

We report the cloning of a cDNA for MEK1, an Arabidopsis thaliana gene encoding a homologue of MAP kinase kinase (MEK). The predicted protein sequence shows 41% identity over 270 amino acids to vertebrate MEK proteins, and contains conserved features characteristic of MEK. Analysis of transcript levels show that expression of the gene is regulated by developmental processes (etiolation/de-etiolation) and by wounding. However in contrast to the rapid wound induction of MAP kinase transcripts in other plant species, MEK1 transcripts first accumulated 6-12 h after wounding.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Base Sequence Blotting, Southern Cloning, Molecular DNA, Complementary/isolation & purification Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genes, Plant Humans MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Plant Proteins/chemistry,genetics,isolation & purification Polymerase Chain Reaction Protein Serine-Threonine Kinases/chemistry,genetics,isolation & purification Protein-Tyrosine Kinases/chemistry,genetics,isolation & purification RNA, Messenger/biosynthesis Sequence Alignment Sequence Homology, Amino Acid
Chemicals
DNA, Complementary Plant Proteins RNA, Messenger Protein-Tyrosine Kinases Protein Serine-Threonine Kinases MAP Kinase Kinase 1 MAP2K1 protein, human Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morris P C
Department of Biological Sciences, Heriot-Watt University, Edinburgh, UK.
Guerrier D
Leung J
Giraudat J
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005963222768
Published
1997-12-00
Pages
1057-64
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AF000977
External Links
PubMed source DOI: 10.1023/a:1005963222768 View journal details
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