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PMID: 9437858 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures.

The Plant cell ·Vol. 9 ·No. 12 ·1997-12-00 ·Pages 2119-34

Barthels N, van der Lee FM, Klap J, Goddijn OJ, Karimi M, Puzio P, Grundler FM, Ohl SA, Lindsey K, Robertson L, Robertson WM, Van Montagu M, Gheysen G, Sijmons PC

Abstract

In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. A large collection of transgenic Arabidopsis plants was generated. They were transformed with a beta-glucuronidase gene functioning as a promoter tag. Three T-DNA constructs, pGV1047, p delta gusBin19, and pMOG553, were used. Early responses to nematode invasion were of primary interest. Six lines exhibiting beta-glucuronidase activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time-course studies revealed that all six tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.

MeSH Terms
Animals Arabidopsis/genetics,parasitology Base Sequence DNA Primers/genetics DNA, Bacterial/genetics Gene Expression Regulation, Plant Genes, Plant Genes, Regulator Genes, Reporter Genetic Vectors Glucuronidase/genetics Nematoda/pathogenicity Plant Diseases/genetics,parasitology Plants, Genetically Modified Polymerase Chain Reaction Promoter Regions, Genetic Transcriptional Activation
Chemicals
DNA Primers DNA, Bacterial T-DNA Glucuronidase
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Barthels N
Department of Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, Belgium.
van der Lee F M
Klap J
Goddijn O J
Karimi M
Puzio P
Grundler F M
Ohl S A
Lindsey K
Robertson L
Robertson W M
Van Montagu M
Gheysen G
Sijmons P C
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.9.12.2119
Published
1997-12-00
Pages
2119-34
Language
English
Region
England
NLM ID
9208688
PMCID
PMC157062
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.9.12.2119 PMC Full Text View journal details
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