Morphological differences in the interactions between human mononuclear cells and coated or uncoated sheep red blood cells: a freeze-etch study of different types of rosettes.

Cell and tissue research ·Vol. 168 ·No. 1 ·1976-04-28 ·Pages 79-88

de Vries E, van de Putte LB, Lafeber GJ, van Buysen-Nutters AC, de Vetten-van der Hulst HL, Meyer CJ

Abstract

Enriched preparations of E, EA and EAC rosettes formed by human peripheral blood mononuclear cells were freeze-etched and examined electron-microscopically. In E rosettes only lymphocyte were involved, whereas in EA and EAC rosettes lymphocytes and mononuclear phagocytes participated as rosette-forming cell. In EA and EAC rosettes, cytoplasmic extensions of the rosette forming cell were seen to penetrate the sheep red blood cell, whereas E rosettes showed a broad zone of adherence without penetration. None of the three types of rosettes showed an interspace between the membranes. Unlike E rosettes, EA and EAC rosettes showed polarity in the adherence of sheep red blood cells. These observations made by freeze-etch electron microscopy indicate distinct morphological differences between rosettes formed with coated or uncoated erythrocytes.

MeSH Terms

Animals Cell Membrane/ultrastructure Complement C3 Erythrocytes/ultrastructure Freeze Etching Humans Immune Adherence Reaction Lymphocytes/immunology,ultrastructure Monocytes/ultrastructure Phagocytes/ultrastructure Receptors, Antigen, B-Cell Sheep/immunology

Chemicals

Complement C3 Receptors, Antigen, B-Cell

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

de Vries E

van de Putte L B

Lafeber G J

van Buysen-Nutters A C

de Vetten-van der Hulst H L

Meyer C J

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18 references, click to expand

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Article Info

Journal

Cell and tissue research

Abbr.

Cell Tissue Res

ISSN

0302-766X

DOI

10.1007/BF00219725

Published

1976-04-28

Pages

79-88

Language

English

Region

Germany

NLM ID

0417625

Subset

External Links

PubMed source DOI: 10.1007/BF00219725 View journal details

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Morphological differences in the interactions between human mononuclear cells and coated or uncoated sheep red blood cells: a freeze-etch study of different types of rosettes.

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