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PMID: 9463391 Published · ppublish English Journal Article

Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis.

The EMBO journal ·Vol. 17 ·No. 4 ·1998-02-16 ·Pages 1141-51

Janosi L, Mottagui-Tabar S, Isaksson LA, Sekine Y, Ohtsubo E, Zhang S, Goon S, Nelken S, Shuda M, Kaji A

Abstract

Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post-termination complex of ribosomes, mRNA and tRNA. We now report the first in vivo evidence of RRF function using 12 temperature-sensitive Escherichia coli mutants which we isolated in this study. At non-permissive temperatures, most of the ribosomes remain on mRNA, scan downstream from the termination codon, and re-initiate translation at various sites in all frames without the presence of an initiation codon. Re-initiation does not occur upstream from the termination codon nor beyond a downstream initiation signal. RRF inactivation was bacteriostatic in the growing phase and bactericidal during the transition between the stationary and growing phase, confirming the essential nature of the fourth step of protein synthesis in vivo.

MeSH Terms
Alleles Amino Acid Sequence Amino Acid Substitution/genetics Bacterial Proteins/biosynthesis,genetics,isolation & purification Base Sequence Chromosomes, Bacterial/genetics Codon/physiology Escherichia coli/metabolism,physiology Molecular Sequence Data Mutagenesis Peptide Chain Initiation, Translational/genetics Peptide Chain Termination, Translational/genetics Phenotype Proteins Ribosomal Proteins Ribosomes/metabolism Temperature
Chemicals
Bacterial Proteins Codon Proteins Ribosomal Proteins ribosome releasing factor
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Janosi L
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Mottagui-Tabar S
Isaksson L A
Sekine Y
Ohtsubo E
Zhang S
Goon S
Nelken S
Shuda M
Kaji A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.4.1141
Published
1998-02-16
Pages
1141-51
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170462
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.4.1141 PMC Full Text View journal details
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